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Molecular & Cellular Proteomics 6:18-28, 2007.
© 2007 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Secretome of Primary Cultures of Human Adipose-derived Stem Cells

Modulation of Serpins by Adipogenesis^*,S

Sanjin Zvonic, Michael Lefevre^,¶, Gail Kilroy^,||, Z. Elizabeth Floyd, James P. DeLany^**, Indu Kheterpal, Amy Gravois, Ryan Dow, Angie White, Xiying Wu and Jeffrey M. Gimble^,||,

From the Stem Cell Laboratory, Proteomic Core Facility, ^¶ Lipoprotein Laboratory, and ^|| Cell Biology Core Facility, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, Louisiana 70808 and ^** Endocrinology and Metabolism Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15213

Studies of adipogenic protein induction have led to a new appreciation of the role of adipose tissue as an endocrine organ. Adipocyte-derived "adipokines" such as adiponectin, leptin, and visceral adipose tissue-derived serine protease inhibitor (vaspin) exert hormone-like activities at the systemic level. In this study, we examined the secretome of primary cultures of human subcutaneous adipose-derived stem cells as an in vitro model of adipogenesis. Conditioned media obtained from four individual female donors after culture in uninduced or adipogenic induced conditions were compared by two-dimensional gel electrophoresis and tandem mass spectrometry. Over 80 individual protein features showing 2-fold relative differences were examined. Approximately 50% of the identified proteins have been described previously in the secretome of murine 3T3-L1 preadipocytes or in the interstitial fluid derived from human mammary gland adipose tissue. As reported by others, we found that the secretome included proteins such as actin and lactate dehydrogenase that do not display a leader sequence or transmembrane domain and are classified as "cytoplasmic" in origin. Moreover we detected a number of established adipokines such as adiponectin and plasminogen activator inhibitor 1. Of particular interest was the presence of multiple serine protease inhibitors (serpins). In addition to plasminogen activator inhibitor 1, these included pigment epithelium-derived factor (confirmed by Western immunoblot), placental thrombin inhibitor, pregnancy zone protein, and protease C1 inhibitor. These findings, together with the recent identification of vaspin, suggest that the serpin protein family warrants further proteomics investigation with respect to the etiology of obesity and type 2 diabetes.

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