HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M600234-MCP200v1 6/1/56    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Glossary Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Cha, M.-H. Articles by Park, C.-S. Search for Related Content PubMed PubMed Citation Articles by Cha, M.-H. Articles by Park, C.-S. Social Bookmarking                      What's this?

---

Research

Proteomic Identification of Macrophage Migration-inhibitory Factor upon Exposure to TiO₂ Particles^*

Myung-Hwa Cha^,, TaiYoun Rhim^,, Kyung Hun Kim, An-Soo Jang^,¶, Young-Ki Paik^|| and Choon-Sik Park^,**

From the Genome Research Center for Allergy and Respiratory Diseases, Soonchunhyang University Hospital, Bucheon-si, Gyeonggi-do 420-853, Korea and ^|| Yonsei Proteome Research Center, Seoul, 120-749, Korea

Inhalation of particulate matter aggravates respiratory symptoms in patients with chronic airway diseases, but the mechanisms underlying this response remain poorly understood. We used a proteomics approach to examine this phenomenon. Treatment of epithelial cells with BSA-coated titanium dioxide (TiO₂) particles altered 20 protein spots on the two-dimensional gel, and these were then analyzed by nano-LC-MS/MS. These proteins included defense-related, cell-activating, and cytoskeletal proteins implicated in the response to oxidative stress. The proteins were classified into four groups according to the time course of their expression patterns. For validation, RT-PCR was performed on extracts of in vitro TiO₂-treated cells, and lung issues from TiO₂-treated rats were analyzed by immunohistochemical staining and enzyme immunoassay. TiO₂ treatment was found to increase the amount of mRNA for macrophage migration-inhibitory factor (MIF). MIF was expressed primarily in epithelium and was elevated in lung tissues and bronchoalveolar lavage fluids of TiO₂-treated rats as compared with sham-treated rats. Carbon black and diesel exhaust particles also induced expression of MIF protein in the epithelial cells.

^** To whom correspondence may be addressed: Div. of Allergy and Respiratory Medicine, Dept. of Internal Medicine, Soonchunhyang University Hospital, 1174 Jung-dong, Wonmi-gu, Bucheon-si, Gyeonggi-do 420-767, Republic of Korea. Tel.: 82-32-621-5105; Fax: 82-32-621-5018; E-mail: mdcspark{at}unitel.co.kr

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?