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Molecular & Cellular Proteomics 6:1933-1941, 2007.
© 2007 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Enhanced N-Glycosylation Site Analysis of Sialoglycopeptides by Strong Cation Exchange Prefractionation Applied to Platelet Plasma Membranes ^*,S

Urs Lewandrowski^,, René P. Zahedi^,, Jan Moebius, Ulrich Walter^¶ and Albert Sickmann

From the DFG Research Center for Experimental Biomedicine, University Würzburg, Versbacher Strasse 9, 97078 Würzburg, Germany and ^¶ Department of Clinical Biochemistry and Pathobiochemistry, University Würzburg, Joseph Schneider Strasse 2, 97080 Wzburg, Germany

Elucidation of post-translational modifications to proteins, such as glycosylations or phosphorylations, is one of the major issues concerning ongoing proteomics studies. To reduce general sample complexity, a necessary prerequisite is specific enrichment of peptide subsets prior to mass spectrometric sequencing. Regarding analysis of overall N-glycosylation sites in the past, this has been achieved by several approaches proving to be more or less complicated and specific. Here we present a novel strategy to target N-glycosylation sites with application to platelet membrane proteins. Initial aqueous two-phase partitioning for membrane enrichment and single step strong cation exchange-based purification of glycopeptides resulted in identification of 148 glycosylation sites on 79 different protein species. Although 69% of these sites were not annotated in the Swiss-Prot database before, a high number of 75% plasma membrane-localized proteins were analyzed. Furthermore miniaturizations and relative quantification are comprised in the developed method suggesting further use in other proteome projects. Results on platelet glycosylation sites may imply an impact on research of bleeding disorders as well as potential new functions in inflammation and immunoactivity.

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				U. Lewandrowski, S. Wortelkamp, K. Lohrig, R. P. Zahedi, D. A. Wolters, U. Walter, and A. Sickmann Platelet membrane proteomics: a novel repository for functional research Blood, July 2, 2009; 114(1): e10 - e19. [Abstract] [Full Text] [PDF]