HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M700123-MCP200v1 6/12/2058    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Glossary Citing Articles Citing Articles via HighWire Google Scholar Articles by Deng, Z. Articles by Wang, Z.-Y. PubMed PubMed Citation Articles by Deng, Z. Articles by Wang, Z.-Y.

---

Research

A Proteomics Study of Brassinosteroid Response in Arabidopsis^*,S

Zhiping Deng, Xin Zhang, Wenqiang Tang, Juan A. Oses-Prieto, Nagi Suzuki, Joshua M. Gendron^,¶,||, Huanjing Chen, Shenheng Guan, Robert J. Chalkley, T. Kaye Peterman^**, Alma L. Burlingame and Zhi-Yong Wang^,

From the Department of Plant Biology, Carnegie Institution of Washington, Stanford, California 94305, Mass Spectrometry Facility, Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143, ^¶ Department of Biological Sciences, Stanford University, Stanford, California 94305, and ^** Department of Biological Sciences, Wellesley College, Wellesley, Massachusetts 02481

The plant steroid hormones brassinosteroids (BRs) play an important role in a wide range of developmental and physiological processes. How BR signaling regulates diverse processes remains unclear. To understand the molecular details of BR responses, we performed a proteomics study of BR-regulated proteins in Arabidopsis using two-dimensional DIGE coupled with LC-MS/MS. We identified 42 BR-regulated proteins, which are predicted to play potential roles in BR regulation of specific cellular processes, such as signaling, cytoskeleton rearrangement, vesicle trafficking, and biosynthesis of hormones and vitamins. Analyses of the BR-insensitive mutant bri1-116 and BR-hypersensitive mutant bzr1-1D identified five proteins (PATL1, PATL2, THI1, AtMDAR3, and NADP-ME2) affected both by BR treatment and in the mutants, suggesting their importance in BR action. Selected proteins were further studied using insertion knock-out mutants or immunoblotting. Interestingly about 80% of the BR-responsive proteins were not identified in previous microarray studies, and direct comparison between protein and RNA changes in BR mutants revealed a very weak correlation. RT-PCR analysis of selected genes revealed gene-specific kinetic relationships between RNA and protein responses. Furthermore BR-regulated posttranslational modification of BiP2 protein was detected as spot shifts in two-dimensional DIGE. This study provides novel insights into the molecular networks that link BR signaling to specific cellular and physiological responses.

This article has been cited by other articles:

				W. Tang, Z. Deng, J. A. Oses-Prieto, N. Suzuki, S. Zhu, X. Zhang, A. L. Burlingame, and Z.-Y. Wang Proteomics Studies of Brassinosteroid Signal Transduction Using Prefractionation and Two-dimensional DIGE Mol. Cell. Proteomics, April 1, 2008; 7(4): 728 - 738. [Abstract] [Full Text] [PDF]

				J. M. Gendron, A. Haque, N. Gendron, T. Chang, T. Asami, and Z.-Y. Wang Chemical Genetic Dissection of Brassinosteroid-Ethylene Interaction Mol Plant, March 1, 2008; 1(2): 368 - 379. [Abstract] [Full Text] [PDF]