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Originally published In Press as doi:10.1074/mcp.M600346-MCP200 on February 7, 2007.
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Molecular & Cellular Proteomics 6:798-811, 2007.
© 2007 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Molecular Composition of IMP1 Ribonucleoprotein Granules*,S

Lars Jønson{ddagger}, Jonas Vikesaa{ddagger}, Anders Krogh§, Lars K. Nielsen, Thomas vO. Hansen{ddagger}, Rehannah Borup{ddagger}, Anders H. Johnsen{ddagger}, Jan Christiansen||,** and Finn C. Nielsen{ddagger},**,{ddagger}{ddagger}

From the {ddagger} Department of Clinical Biochemistry, Rigshospitalet, || Institute of Molecular Biology, and § Bioinformatics Centre, University of Copenhagen, DK-2100 Copenhagen, Denmark and Danish Fundamental Metrology, Danish Technical University, DK-2800 Lyngby, Denmark

Localized mRNAs are transported to sites of local protein synthesis in large ribonucleoprotein (RNP) granules, but their molecular composition is incompletely understood. Insulin-like growth factor II mRNA-binding protein (IMP) zip code-binding proteins participate in mRNA localization, and in motile cells IMP-containing granules are dispersed around the nucleus and in cellular protrusions. We isolated the IMP1-containing RNP granules and found that they represent a unique RNP entity distinct from neuronal hStaufen and/or fragile X mental retardation protein granules, processing bodies, and stress granules. Granules were 100–300 nm in diameter and consisted of IMPs, 40 S ribosomal subunits, shuttling heterologous nuclear RNPs, poly(A)-binding proteins, and mRNAs. Moreover granules contained CBP80 and factors belonging to the exon junction complex and lacked eIF4E, eIF4G, and 60 S ribosomal subunits, indicating that embodied mRNAs are not translated. Granules embodied mRNAs corresponding to about 3% of the human embryonic kidney 293 mRNA transcriptome. Messenger RNAs encoding proteins participating in the secretory pathway and endoplasmic reticulum-associated quality control, as well as ubiquitin-dependent metabolism, were enriched in the granules, reinforcing the concept of RNP granules as post-transcriptional operons.

{ddagger}{ddagger} To whom correspondence should be addressed. Tel.: 45-3545-2223; Fax: 45-3545-4640, E-mail: FCN{at}rh.dk


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eLetters:

Read all eLetters

FMRP and IMP1: Evidence of Absence or Absence of Evidence, Noise in the Protein Interactome
Robert B. Denman
MCP Online, 2 Apr 2007 [Full text]
Response to Denman Letter
Finn C. Nielsen, et al.
MCP Online, 2 Apr 2007 [Full text]

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