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Molecular & Cellular Proteomics 6:1560-1573, 2007.
© 2007 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Quantitative Profile of Five Murine Core Proteomes Using Label-free Functional Proteomics^*,S

Pedro R. Cutillas^,,¶,|| and Bart Vanhaesebroeck^,¶,**

From the Cell Signalling Group, Ludwig Institute for Cancer Research, 91 Riding House Street, London W1W 7BS, United Kingdom, ^** Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom, and Proteomics Unit, Ludwig Institute for Cancer Research, Cruciform Building, Gower Street, London WC1E 6BT, United Kingdom

Analysis of primary animal and human tissues is key in biological and biomedical research. Comparative proteomics analysis of primary biological material would benefit from uncomplicated experimental work flows capable of evaluating an unlimited number of samples. In this report we describe the application of label-free proteomics to the quantitative analysis of five mouse core proteomes. We developed a computer program and normalization procedures that allow exploitation of the quantitative data inherent in LC-MS/MS experiments for relative and absolute quantification of proteins in complex mixtures. Important features of this approach include (i) its ability to compare an unlimited number of samples, (ii) its applicability to primary tissues and cultured cells, (iii) its straightforward work flow without chemical reaction steps, and (iv) its usefulness not only for relative quantification but also for estimation of absolute protein abundance. We applied this approach to quantitatively characterize the most abundant proteins in murine brain, heart, kidney, liver, and lung. We matched 8,800 MS/MS peptide spectra to 1,500 proteins and generated 44,000 independent data points to profile the 1,000 most abundant proteins in mouse tissues. This dataset provides a quantitative profile of the fundamental proteome of a mouse, identifies the major similarities and differences between organ-specific proteomes, and serves as a paradigm of how label-free quantitative MS can be used to characterize the phenotype of mammalian primary tissues at the molecular level.

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