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Originally published In Press as doi:10.1074/mcp.M700115-MCP200 on October 13, 2007.
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Molecular & Cellular Proteomics 7:163-169, 2008.
© 2008 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Protein Expression Profiling of Breast Cancer Cells by Dissociable Antibody Microarray (DAMA) Staining*,S

X. Cynthia Song{ddagger},§, Guanyuan Fu{ddagger},§, Xufen Yang{ddagger}, Zhong Jiang, Yingjian Wang|| and G. Wayne Zhou{ddagger},**

From the {ddagger} Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, the Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, and || Hypromatrix Inc., Worcester, Massachusetts 01606

Dissociable antibody microarray (DAMA) staining is a technology that combines protein microarrays with traditional immunostaining techniques. It can simultaneously determine the expression and subcellular location of hundreds of proteins in cultured cells and tissue samples. We developed this technology and demonstrated its application in identifying potential biomarkers for breast cancer. We compared the expression profiles of 312 proteins among three normal breast cell lines and seven breast cancer cell lines and identified 10 differentially expressed proteins by the data analysis program DAMAPEP (DAMA protein expression profiling). Among those proteins, RAIDD, Rb p107, Rb p130, SRF, and Tyk2 were confirmed by Western blot and statistical analysis to have higher expression levels in breast cancer cells than in normal breast cells. These proteins could be potential biomarkers for the diagnosis of breast cancer.

** To whom correspondence should be addressed. Tel.: 225-578-6733; Fax: 225-578-8011; E-mail: zhouw{at}lsu.edu






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