HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) A correction has been published All Versions of this Article: M700544-MCP200v1 7/10/1810    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Li, Z. Articles by Huang, C. Search for Related Content PubMed PubMed Citation Articles by Li, Z. Articles by Huang, C. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 7:1810-1823, 2008.
© 2008 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Proteomics Identification of Cyclophilin A as a Potential Prognostic Factor and Therapeutic Target in Endometrial Carcinoma^*

Zhengyu Li, Xia Zhao, Shujun Bai, Zhi Wang, Lijuan Chen, Yuquan Wei and Canhua Huang

From the Department of Gynecology and Obstetrics and the State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China

Endometrial carcinoma is one of the most common malignancies of the female genital tract, and there is an urgent need for discovery of novel factors for prognostic assessment and therapeutic targets to endometrial carcinoma. Herein a two-dimensional gel electrophoresis and MALDI-Q-TOF MS/MS-based proteomics approach was used to identify differentially expressed proteins in endometrial carcinoma. Of the 99 proteins identified, cyclophilin A was one of the most significantly altered proteins, and its overexpression was confirmed using RT-PCR and Western blot analyses. Immunohistochemistry suggested a link between cyclophilin A expression and poor differentiation and decreased survival (p < 0.01). Knockdown of cyclophilin A expression by RNA interference led to the significant suppression of the cell growth and the induction of apoptosis in endometrial carcinoma HEC-1-B cells in vitro (p < 0.01) and the inhibition of tumor growth in vivo (p < 0.01). These data suggest that cyclophilin A may serve as a novel prognostic factor and possibly an attractive therapeutic target for endometrial carcinoma.

To whom correspondence may be addressed: State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China. Tel.: 86-28-13258370346; Fax: 86-28-85164060; E-mail: hcanhua{at}hotmail.com

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Kreth, J. Heyn, S. Grau, H. A. Kretzschmar, R. Egensperger, and F. W. Kreth Identification of valid endogenous control genes for determining gene expression in human glioma Neuro Oncology, February 5, 2010; (2010) nop072v1. [Abstract] [Full Text] [PDF]

				Y. Ma, J. Peng, W. Liu, P. Zhang, L. Huang, B. Gao, T. Shen, Y. Zhou, H. Chen, Z. Chu, et al. Proteomics Identification of Desmin as a Potential Oncofetal Diagnostic and Prognostic Biomarker in Colorectal Cancer Mol. Cell. Proteomics, August 1, 2009; 8(8): 1878 - 1890. [Abstract] [Full Text] [PDF]