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This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M800264-MCP200v1 7/10/2028    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Glossary Google Scholar Articles by Björling, E. Articles by Uhlén, M. PubMed PubMed Citation Articles by Björling, E. Articles by Uhlén, M. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 7:2028-2037, 2008.
© 2008 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Antibodypedia, a Portal for Sharing Antibody and Antigen Validation Data^*,S

Erik Björling and Mathias Uhlén

From the School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Center, SE-106 91 Stockholm, Sweden

Antibodies are useful tools to characterize the components of the human proteome and to validate potential protein biomarkers discovered through various clinical proteomics efforts. The lack of validation results across various applications for most antibodies often makes it necessary to perform cumbersome investigations to ensure specificity of a particular antibody in a certain application. A need therefore exists for a standardized system for sharing validation data about publicly available antibodies and to allow antibody providers as well as users to contribute and edit experimental evidence data, including data also on the antigen. Here we describe a new publicly available portal called Antibodypedia, which has been developed to allow sharing of information regarding validation of antibodies in which providers can submit their own validation results and reliability scores. We report standardized validation criteria and submission rules for applications such as Western blots, protein arrays, immunohistochemistry, and immunofluorescence. The contributor is expected to provide experimental evidence and a validation score for each antibody, and the users can subsequently provide feedback and comments on the use of the antibody. The database thus provides a virtual resource of publicly available antibodies toward human proteins with accompanying experimental evidence supporting an individual validation score for each antibody in an application-specific manner.

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