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Molecular & Cellular Proteomics 7:499-508, 2008.
© 2008 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Toward a Confocal Subcellular Atlas of the Human Proteome^*,S

Laurent Barbe, Emma Lundberg, Per Oksvold, Anna Stenius, Erland Lewin, Erik Björling, Anna Asplund^¶, Fredrik Pontén^¶, Hjalmar Brismar, Mathias Uhlén^,|| and Helene Andersson-Svahn

From the Departments of Biotechnology and Cell Physics, AlbaNova University Center, Royal Institute of Technology, SE-106 91 Stockholm, Sweden and ^¶ Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE-75185 Uppsala, Sweden

Information on protein localization on the subcellular level is important to map and characterize the proteome and to better understand cellular functions of proteins. Here we report on a pilot study of 466 proteins in three human cell lines aimed to allow large scale confocal microscopy analysis using protein-specific antibodies. Approximately 3000 high resolution images were generated, and more than 80% of the analyzed proteins could be classified in one or multiple subcellular compartment(s). The localizations of the proteins showed, in many cases, good agreement with the Gene Ontology localization prediction model. This is the first large scale antibody-based study to localize proteins into subcellular compartments using antibodies and confocal microscopy. The results suggest that this approach might be a valuable tool in conjunction with predictive models for protein localization.

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