MCP Tips for better browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1074/mcp.M700292-MCP200 on December 14, 2007.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M700292-MCP200v1
7/3/573    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Glossary
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Spooncer, E.
Right arrow Articles by Whetton, A. D.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Spooncer, E.
Right arrow Articles by Whetton, A. D.
Molecular & Cellular Proteomics 7:573-581, 2008.
© 2008 by The American Society for Biochemistry and Molecular Biology, Inc.


Research

Developmental Fate Determination and Marker Discovery in Hematopoietic Stem Cell Biology Using Proteomic Fingerprinting*,S

Elaine Spooncer{ddagger}, Nathalie Brouard§, Susie K. Nilsson§,||, Brenda Williams§,||, Mira C. Liu§, Richard D. Unwin{ddagger}, David Blinco{ddagger}, Ewa Jaworska{ddagger}, Paul J. Simmons§ and Anthony D. Whetton{ddagger},**

From the {ddagger} Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Kinnaird House, Kinnaird Road, Manchester M20 4QL, United Kingdom and § Stem Cell Laboratory, Peter MacCallum Cancer Centre, St. Andrews Place, East Melbourne, Victoria 3002, Australia

In hematopoiesis, co-expression of Sca-1 and c-Kit defines cells (LS+K) with long term reconstituting potential. In contrast, poorly characterized LSK cells fail to reconstitute lethally irradiated recipients. Relative quantification mass spectrometry and transcriptional profiling were used to characterize LS+K and LSK cells. This approach yielded data on >1200 proteins. Only 32% of protein changes correlated to mRNA modulation demonstrating post-translational protein regulation in early hematopoietic development. LS+K cells had lower expression of protein synthesis proteins but did express proteins associated with mature cell function. Major increases in erythroid development proteins were observed in LSK cells; based on this assessment of erythroid potential we showed them to be principally erythroid progenitors, demonstrating effective use of discovery proteomics for definition of primitive cells.


** To whom correspondence should be addressed. Tel.: 44-161-446-8247; Fax: 44-161-446-8203; E-mail: anthony.whetton{at}manchester.ac.uk







HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2008 by the American Society for Biochemistry and Molecular Biology.