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Originally published In Press as doi:10.1074/mcp.M700483-MCP200 on March 14, 2008.
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Molecular & Cellular Proteomics 7:1174-1185, 2008.
© 2008 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Quantitative and Temporal Proteome Analysis of Butyrate-treated Colorectal Cancer Cells*,S

Hwee Tong Tan{ddagger}, Sandra Tan§, Qingsong Lin§, Teck Kwang Lim§, Choy Leong Hew§ and Maxey C. M. Chung{ddagger},§,¶

From the {ddagger} Department of Biochemistry, Yong Loo Lin School of Medicine and § Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 117597, Singapore

Colorectal cancer is one of the most common cancers in developed countries, and its incidence is negatively associated with high dietary fiber intake. Butyrate, a short-chain fatty acid fermentation by-product of fiber induces cell maturation with the promotion of growth arrest, differentiation, and/or apoptosis of cancer cells. The stimulation of cell maturation by butyrate in colonic cancer cells follows a temporal progression from the early phase of growth arrest to the activation of apoptotic cascades. Previously we performed two-dimensional DIGE to identify differentially expressed proteins induced by 24-h butyrate treatment of HCT-116 colorectal cancer cells. Herein we used quantitative proteomics approaches using iTRAQ (isobaric tags for relative and absolute quantitation), a stable isotope labeling methodology that enables multiplexing of four samples, for a temporal study of HCT-116 cells treated with butyrate. In addition, cleavable ICAT, which selectively tags cysteine-containing proteins, was also used, and the results complemented those obtained from the iTRAQ strategy. Selected protein targets were validated by real time PCR and Western blotting. A model is proposed to illustrate our findings from this temporal analysis of the butyrate-responsive proteome that uncovered several integrated cellular processes and pathways involved in growth arrest, apoptosis, and metastasis. These signature clusters of butyrate-regulated pathways are potential targets for novel chemopreventive and therapeutic drugs for treatment of colorectal cancer.

To whom correspondence should be addressed: Dept. of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 117597, Singapore. Tel.: 65-65163252; Fax: 65-67791453; E-mail: bchcm{at}nus.edu.sg


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