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Originally published In Press as doi:10.1074/mcp.M700458-MCP200 on March 14, 2008.
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Molecular & Cellular Proteomics 7:1317-1330, 2008.
© 2008 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Deep Coverage Mouse Red Blood Cell Proteome

A First Comparison with the Human Red Blood Cell*,S

Erica M. Pasini{ddagger}, Morten Kirkegaard§, Doris Salerno{ddagger}, Peter Mortensen§, Matthias Mann and Alan W. Thomas{ddagger},||

From the {ddagger} Biomedical Primate Research Centre, Lange Kleiweg 139, 2288 GJ Rijswijk, The Netherlands, § Center for Experimental Bioinformatics, University of Southern Denmark, Campusvej 55, 5320 Odense, Denmark, and the Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany

Mice have close genetic/physiological relationships to humans, breed rapidly, and can be genetically modified, making them the most used mammal in biomedical research. Because the red blood cell (RBC) is the sole gas transporter in vertebrates, diseases of the RBC are frequently severe; much research has therefore focused on RBC and cardiovascular disorders of mouse and humans. RBCs also host malaria parasites. Recently we presented an in-depth proteome for the human RBC. Here we present directly comparable data for the mouse RBC as membrane-only, soluble-only, and combined membrane-bound/soluble proteomes (comprising, respectively, 247, 232, and 165 proteins). All proteins were identified, validated, and categorized in terms of subcellular localization, protein family, and function, and in comparison with the human RBC, were classified as orthologs, family-related, or unique. Splice isoforms were identified, and polypeptides migrating with anomalous apparent molecular weights were grouped into putatively ubiquitinated or partially degraded complexes. Overall there was close concordance between mouse and human proteomes, confirming the unexpected RBC complexity. Several novel findings in the human proteome have been confirmed here. This comparison sheds light on several open issues in RBC biology and provides a departure point for more comprehensive understanding of RBC function.

|| To whom correspondence should be addressed. Tel.: 31-15-284-2538; Fax: 31-15-284-2600; E-mail: thomas{at}bprc.nl


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