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Molecular & Cellular Proteomics 7:1501-1516, 2008.
© 2008 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Cross-species Global Proteomics Reveals Conserved and Unique Processes in Phytophthora sojae and Phytophthora ramorum^*,S

Alon Savidor, Ryan S. Donahoo, Oscar Hurtado-Gonzales, Miriam L. Land^¶, Manesh B. Shah^¶, Kurt H. Lamour and W. Hayes McDonald^||,**

From the Graduate School of Genome Science and Technology, University of Tennessee-Oak Ridge National Laboratory Oak Ridge, Oak Ridge, Tennessee 37830, Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, Tennessee 37996-4560, ^¶ Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, and ^|| Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831

Phytophthora ramorum and Phytophthora sojae are destructive plant pathogens. P. sojae has a narrow host range, whereas P. ramorum has a wide host range. A global proteomics comparison of the vegetative (mycelium) and infective (germinating cyst) life stages of P. sojae and P. ramorum was conducted to identify candidate proteins involved in host range, early infection, and vegetative growth. Sixty-two candidates for early infection, 26 candidates for vegetative growth, and numerous proteins that may be involved in defining host specificity were identified. In addition, common life stage proteomic trends between the organisms were observed. In mycelia, proteins involved in transport and metabolism of amino acids, carbohydrates, and other small molecules were up-regulated. In the germinating cysts, up-regulated proteins associated with lipid transport and metabolism, cytoskeleton, and protein synthesis were observed. It appears that the germinating cyst catabolizes lipid reserves through the β-oxidation pathway to drive the extensive protein synthesis necessary to produce the germ tube and initiate infection. Once inside the host, the pathogen switches to vegetative growth in which energy is derived from glycolysis and utilized for synthesis of amino acids and other molecules that assist survival in the plant tissue.

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