HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M800156-MCP200v1 8/1/19    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lee, J. Articles by Cooper, B. Search for Related Content PubMed PubMed Citation Articles by Lee, J. Articles by Cooper, B. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 8:19-31, 2009.
© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Quantitative Proteomic Analysis of Bean Plants Infected by a Virulent and Avirulent Obligate Rust Fungus ^*,S

Joohyun Lee^,, Jian Feng^¶, Kimberly B. Campbell, Brian E. Scheffler^||, Wesley M. Garrett^**, Sandra Thibivilliers, Gary Stacey, Daniel Q. Naiman^¶, Mark L. Tucker, M. A. Pastor-Corrales and Bret Cooper^,

From the Soybean Genomics and Improvement Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705, ^¶ Department of Applied Mathematics and Statistics, The Johns Hopkins University, Baltimore, Maryland 21218, ^|| Mid South Area Genomics Laboratory, United States Department of Agriculture, Agricultural Research Service, Stoneville, Mississippi 38776, ^** Animal Biosciences and Biotechnology Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705, and Divisions of Plant Sciences and Biochemistry, University of Missouri, Columbia, Missouri 65211

Plants appear to have two types of active defenses, a broad-spectrum basal system and a system controlled by R-genes providing stronger resistance to some pathogens that break the basal defense. However, it is unknown if the systems are separate entities. Therefore, we analyzed proteins from leaves of the dry bean crop plant Phaseolus vulgaris using a high-throughput liquid chromatography tandem mass spectrometry method. By statistically comparing the amounts of proteins detected in a single plant variety that is susceptible or resistant to infection, depending on the strains of a rust fungus introduced, we defined basal and R-gene-mediated plant defenses at the proteomic level. The data reveal that some basal defense proteins are potential regulators of a strong defense weakened by the fungus and that the R-gene modulates proteins similar to those in the basal system. The results satisfy a new model whereby R-genes are part of the basal system and repair disabled defenses to reinstate strong resistance.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?