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This Article Full Text Full Text (PDF) Supplemental Data Supplemental Data All Versions of this Article: M900194-MCP200v1 8/10/2396    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Google Scholar Articles by Birkó, Z. Articles by Biró, S. PubMed PubMed Citation Articles by Birkó, Z. Articles by Biró, S. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 8:2396-2403, 2009.
© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Lack of A-factor Production Induces the Expression of Nutrient Scavenging and Stress-related Proteins in Streptomyces griseus^*,

Zsuzsanna Birkó, Magdalena Swiatek, Emília Szájli^¶, Katalin F. Medzihradszky^¶,||, Erik Vijgenboom, András Penyige, Judit Keser, Gilles P. van Wezel^,** and Sándor Biró^,

From the Department of Human Genetics, Faculty of Medicine, Medical and Health Science Center, University of Debrecen, Nagyerdei körút 98, H-4032 Debrecen, Hungary,
¶Proteomics Research Group, Biological Research Center, Hungarian Academy of Sciences, Temesvári körút 62, H-6726 Szeged, Hungary,
||Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94158-2517, and
Microbial Development, Leiden Institute of Chemistry, Leiden University, Gorlaeus Laboratories, P. O. Box 9502, 2300 RA Leiden, The Netherlands

The small -butyrolactone A-factor is an important autoregulatory signaling molecule for the soil-inhabiting streptomycetes. Starvation is a major trigger for development, and nutrients are provided by degradation of the vegetative mycelium via a process of programmed cell death, reusing proteins, nucleic acids, and cell wall material. The A-factor regulon includes many extracellular hydrolases. Here we show via proteomics analysis that many nutrient-scavenging and stress-related proteins were overexpressed in an A-factor non-producing mutant of Streptomyces griseus B-2682. Transcript analysis showed that this is primarily due to differential transcription of the target genes during early development. The targets include proteins relating to nutrient stress and environmental stress and an orthologue of the Bacillus sporulation control protein Spo0M. The enhanced expression of these proteins underlines the stress that is generated by the absence of A-factor. Wild-type developmental gene expression was restored to the A-factor non-producing mutant by the signaling protein Factor C in line with our earlier observation that Factor C triggers A-factor production.

To whom correspondence may be addressed. Tel./Fax:36-52416531; E-mail:sbiro{at}med.unideb.hu.

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