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This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M800268-MCP200v1 8/4/780    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Trzcinska-Daneluti, A. M. Articles by Rotin, D. Search for Related Content PubMed PubMed Citation Articles by Trzcinska-Daneluti, A. M. Articles by Rotin, D. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 8:780-790, 2009.
© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

High-content Functional Screen to Identify Proteins that Correct F508del-CFTR Function^*,S

Agata M. Trzciska-Daneluti, Diane Ly, Lise Huynh, Chong Jiang, Christopher Fladd and Daniela Rotin

From the Program in Cell Biology, The Hospital for Sick Children, and Biochemistry Department, University of Toronto, Toronto, Ontario M5G 1L7, Canada

Cystic Fibrosis is caused by mutations in CFTR, with a deletion of a phenylalanine at position 508 (F508del-CFTR) representing the most common mutation. The F508del-CFTR protein exhibits a trafficking defect and is retained in the endoplasmic reticulum. Here we describe the development of a high-content screen based on a functional assay to identify proteins that correct the F508del-CFTR defect. Using a HEK293 MSR GripTite cell line that stably expresses F508del-CFTR, we individually co-expressed 450 unique proteins fused to the Cl^–-sensitive YFP(H148Q/I152L) mutant. We then tested correction of F508del-CFTR function by the CI^–/l^– exchange method following stimulation with forskolin/IBMX/genistein, using quantitative recordings in multiple individual cells with a high-content (high-throughput) Cellomics KSR imaging system. Using this approach, we identified several known and novel proteins that corrected F508del-CFTR function, including STAT1, Endothelin 1, HspA4, SAPK substrate protein 1, AP2M1, LGALS3/galectin-3, Trk-fused gene, Caveolin 2, PAP/REG3, and others. The ability of these correctors to rescue F508del-CFTR trafficking was then validated by demonstrating their enhancement of maturation (appearance of band C) and by cell surface expression of F508del-CFTR bearing HA tag at the ectodomain using confocal microscopy and flow cytometry. These data demonstrate the utility of high-content analyses for identifying proteins that correct mutant CFTR and discover new proteins that stimulate this correction. This assay can also be utilized for RNAi screens to identify inhibitory proteins that block correction of F508del-CFTR, small molecule, and peptide screens.

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