HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M800422-MCP200v1 8/5/1105    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Poschmann, G. Articles by Stühler, K. Search for Related Content PubMed PubMed Citation Articles by Poschmann, G. Articles by Stühler, K. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 8:1105-1116, 2009.
© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Identification of Proteomic Differences between Squamous Cell Carcinoma of the Lung and Bronchial Epithelium^*,S

Gereon Poschmann, Barbara Sitek, Bence Sipos, Anna Ulrich, Sebastian Wiese, Christian Stephan, Bettina Warscheid, Günter Klöppel, Ann Vander Borght^¶, Frans C. S. Ramaekers^¶, Helmut E. Meyer and Kai Stühler^,||

From the Medizinisches Proteom-Center, Ruhr-Universität Bochum, 44801 Bochum, Germany, Department of Pathology, University of Kiel, 24105 Kiel, Germany, and ^¶ Department of Molecular Cell Biology, GROW-School for Oncology and Developmental Biology University of Maastricht and MUbio Products B.V., Maastricht, 6200 MD Maastricht, The Netherlands

Proteins that exhibit different expression levels in normal and malignant lung cells are good candidate biomarkers to improve early diagnosis and intervention. We used a quantitative approach and compared the proteome of microdissected cells from normal human bronchial epithelium and squamous cell carcinoma tumors of histopathological grades G2 and G3. DIGE analysis and subsequent MS-based protein identification revealed that 32 non-redundant proteins were differentially regulated between the respective tissue types. These proteins are mainly involved in energy pathways, cell growth or maintenance mechanisms, protein metabolism, and the regulation of DNA and RNA metabolism. The expression of some of these proteins was analyzed by immunohistochemistry using tissue microarrays containing tissue specimen of 55 patients, including normal bronchial epithelium, squamous cell carcinomas, adenocarcinomas, and large cell carcinomas. The results of the immunohistochemical studies correlated with the proteome study data and revealed that particularly HSP47 and a group of cytokeratins (i.e. cytokeratins 6a, 16, and 17) are significantly co-regulated in squamous cell carcinoma. Furthermore cytokeratin 17 showed significantly higher abundance in G2 grade compared with G3 grade squamous cell carcinomas in both the gel-based and the immunohistochemical analysis. Therefore this protein might be used as a marker for stratification between different tumor grades.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?