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Molecular & Cellular Proteomics 8:883-886, 2009.
© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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Review

A Human Proteome Detection and Quantitation Project^*

N. Leigh Anderson^,, Norman G. Anderson, Terry W. Pearson^¶, Christoph H. Borchers^||, Amanda G. Paulovich^**, Scott D. Patterson, Michael Gillette, Ruedi Aebersold^¶¶ and Steven A. Carr

From the The Plasma Proteome Institute, Washington, D. C. 20009, ^¶ Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada, ^|| University of Victoria-Genome BC Proteomics Centre, Vancouver Island Technology Park, Victoria, British Columbia V8Z 7X8, Canada, ^** Fred Hutchinson Cancer Research Center, Seattle, Washington WA 98109-1024, Amgen Inc., California 91320-1799, The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, and ^¶¶ Institute of Molecular Systems Biology, HPTE 78, Wolfgang-Pauli-Str. 16, 8093 Zurich, Switzerland

The lack of sensitive, specific, multiplexable assays for most human proteins is the major technical barrier impeding development of candidate biomarkers into clinically useful tests. Recent progress in mass spectrometry-based assays for proteotypic peptides, particularly those with specific affinity peptide enrichment, offers a systematic and economical path to comprehensive quantitative coverage of the human proteome. A complete suite of assays, e.g. two peptides from the protein product of each of the 20,500 human genes (here termed the human Proteome Detection and Quantitation project), would enable rapid and systematic verification of candidate biomarkers and lay a quantitative foundation for subsequent efforts to define the larger universe of splice variants, post-translational modifications, protein-protein interactions, and tissue localization.

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