HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M800589-MCP200v1 8/6/1252    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Villiers, C. Articles by Rabilloud, T. Search for Related Content PubMed PubMed Citation Articles by Villiers, C. Articles by Rabilloud, T. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 8:1252-1264, 2009.
© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

From Secretome Analysis to Immunology

CHITOSAN INDUCES MAJOR ALTERATIONS IN THE ACTIVATION OF DENDRITIC CELLS VIA A TLR4-DEPENDENT MECHANISM

Christian Villiers^,, Mireille Chevallet^,¶,||, Hélène Diemer^**, Rachel Couderc, Heidi Freitas, Alain Van Dorsselaer^**, Patrice N. Marche and Thierry Rabilloud^¶,||,

From the INSERM U823 Analytical Immunology of Chronic Pathologies, Institut Albert Bonniot, BP170, 38042 Grenoble, France and Université Joseph Fourier, F-38041 Grenoble, France,
¶Commissariat à l'Energie Atomique (CEA)-Direction des Sciences du Vivant/Institut de Recherches en Technologies et Sciences pour le Vivant (iRTSV)/Laboratoire de Biochimie et Biophysique des Systèmes Intégrés (LBBSI), Biophysique et Biochimie des Systèmes Intégrés and
||CNRS UMR5092, Biophysique et Biochimie des Systèmes Intégrés, CEA-Grenoble, 17 rue des martyrs, F-38054 Grenoble Cedex 9, France, and
**CNRS UMR7178, Institut Pluridisciplinaire Hubert Curien, Ecole de Chimie, Polymères et Matériaux, 25 rue Becquerel, F-67087 Strasbourg Cedex 2, France

Dendritic cells are known to be activated by a wide range of microbial products, leading to cytokine production and increased levels of membrane markers such as major histocompatibility complex class II molecules. Such activated dendritic cells possess the capacity to activate naïve T cells. In the present study we demonstrated that immature dendritic cells secrete both the YM1 lectin and lipocalin-2. By testing the ligands of these two proteins, chitosan and siderophores, respectively, we also demonstrated that chitosan, a degradation product of various fungal and protozoal cell walls, induces an activation of dendritic cells at the membrane level, as shown by the up-regulation of membrane proteins such as class II molecules, CD80 and CD86 via a TLR4-dependent mechanism, but is not able to induce cytokine production. This led to the production of activated dendritic cells unable to stimulate T cells. However, costimulation with other microbial products overcame this partial activation and restored the capacity of these activated dendritic cells to stimulate T cells. In addition, successive stimulation with chitosan and then by lipopolysaccharide induced a dose-dependent change in the cytokinic IL-12/IL-10 balance produced by the dendritic cells.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?