HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M800546-MCP200v1 8/6/1350    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Commichau, F. M. Articles by Stülke, J. Search for Related Content PubMed PubMed Citation Articles by Commichau, F. M. Articles by Stülke, J. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 8:1350-1360, 2009.
© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Novel Activities of Glycolytic Enzymes in Bacillus subtilis

INTERACTIONS WITH ESSENTIAL PROTEINS INVOLVED IN mRNA PROCESSING^*,

Fabian M. Commichau^,, Fabian M. Rothe, Christina Herzberg, Eva Wagner, Daniel Hellwig, Martin Lehnik-Habrink, Elke Hammer^¶, Uwe Völker^¶ and Jörg Stülke^,,||

From the Department of General Microbiology, Georg-August-University Göttingen, Grisebachstrasse 8, D-37077 Göttingen, Germany and
¶Interfaculty Institute for Genetics and Functional Genomics, Ernst-Moritz-Arndt-University Greifswald, Friedrich-Ludwig-Jahnstrasse 15a, D-17487 Greifswald, Germany

Glycolysis is one of the most important metabolic pathways in heterotrophic organisms. Several genes encoding glycolytic enzymes are essential in many bacteria even under conditions when neither glycolytic nor gluconeogenic activities are required. In this study, a screening for in vivo interaction partners of glycolytic enzymes of the soil bacterium Bacillus subtilis was used to provide a rationale for essentiality of glycolytic enzymes. Glycolytic enzymes proved to be in close contact with several other proteins, among them a high proportion of essential proteins. Among these essential interaction partners, other glycolytic enzymes were most prominent. Two-hybrid studies confirmed interactions of phosphofructokinase with phosphoglyceromutase and enolase. Such a complex of glycolytic enzymes might allow direct substrate channeling of glycolytic intermediates. Moreover we found associations of glycolytic enzymes with several proteins known or suspected to be involved in RNA processing and degradation. One of these proteins, Rny (YmdA), which has so far not been functionally characterized, is required for the processing of the mRNA of the glycolytic gapA operon. Two-hybrid analyses confirmed the interactions between the glycolytic enzymes phosphofructokinase and enolase and the enzymes involved in RNA processing, RNase J1, Rny, and polynucleotide phosphorylase. Moreover RNase J1 interacts with its homologue RNase J2. We suggest that this complex of mRNA processing and glycolytic enzymes is the B. subtilis equivalent of the RNA degradosome. Our findings suggest that the functional interaction of glycolytic enzymes with essential proteins may be the reason why they are indispensable.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. C. Zweers, T. Wiegert, and J. M. van Dijl Stress-Responsive Systems Set Specific Limits to the Overproduction of Membrane Proteins in Bacillus subtilis Appl. Envir. Microbiol., December 1, 2009; 75(23): 7356 - 7364. [Abstract] [Full Text] [PDF]

				L. A. Florez, S. F. Roppel, A. G. Schmeisky, C. R. Lammers, and J. Stulke A community-curated consensual annotation that is continuously updated: the Bacillus subtilis centred wiki SubtiWiki Database, September 17, 2009; 2009(0): bap012 - bap012. [Abstract] [Full Text] [PDF]