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Molecular & Cellular Proteomics 8:1490-1500, 2009.
© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Evaluation of Blastomere Biopsy Using a Mouse Model Indicates the Potential High Risk of Neurodegenerative Disorders in the Offspring^*,

Yang Yu^,,¶, Jindao Wu^¶,||, Yong Fan, Zhuo Lv, Xuejiang Guo^||, Chun Zhao^||, Rong Zhou^||, Zhuo Zhang^||, Fuqiang Wang^||, Min Xiao^||, Ling Chen^||, Hui Zhu^||, Wen Chen^||, Min Lin^||, Jiayin Liu^||, Zuomin Zhou^||, Liu Wang, Ran Huo^||,**, Qi Zhou^, and Jiahao Sha^||

From the State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China,
||Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China, and
Graduate School, Chinese Academy of Sciences, Beijing 100049, China

Preimplantation genetic diagnosis (PGD), used in clinical practice, is offered to couples that may suffer from a monogenetic disorder, chromosome aneuploidy, or X-linked disease. However, blastomere biopsy, as an indispensable manipulation during the PGD procedure has not been assessed for its long term health implications. Using a mouse model, we investigated the effect of blastomere biopsy of in vitro cultured four-cell embryos on preimplantation development efficiency, postnatal growth, and physiological and behavioral activity compared with control, non-biopsied embryos. The mice generated after blastomere biopsy showed weight increase and some memory decline compared with the control group. Further protein expression profiles in adult brains were analyzed by a proteomics approach. A total of 36 proteins were identified with significant differences between the biopsied and control groups, and the alterations in expression of most of these proteins have been associated with neurodegenerative diseases. Furthermore hypomyelination of the nerve fibers was observed in the brains of mice in the biopsied group. This study suggested that the nervous system may be sensitive to blastomere biopsy procedures and indicated an increased relative risk of neurodegenerative disorders in the offspring generated following blastomere biopsy. Thus, more studies should be performed to address the possible adverse effects of blastomere biopsy on the development of offspring, and the overall safety of PGD technology should be more rigorously assessed.

To whom correspondence may be addressed: Tel.: 86-10-64807299; Fax: 86-10-64807306; E-mail: qzhou{at}ioz.ac.cn.

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