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Molecular & Cellular Proteomics 8:1728-1737, 2009.
© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Surfome Analysis as a Fast Track to Vaccine Discovery

IDENTIFICATION OF A NOVEL PROTECTIVE ANTIGEN FOR GROUP B STREPTOCOCCUS HYPERVIRULENT STRAIN COH1

Francesco Doro, Sabrina Liberatori, Manuel J. Rodríguez-Ortega, Cira D. Rinaudo, Roberto Rosini, Marirosa Mora, Maria Scarselli, Emrah Altindis, Romina D'Aurizio, Maria Stella, Immaculada Margarit, Domenico Maione, John L. Telford, Nathalie Norais and Guido Grandi

From the Research Centre, Novartis Vaccines and Diagnostics, 53100 Siena, Italy

Safe recombinant vaccines, based on a small number of antigenic proteins, are emerging as the most attractive, cost-effective solution against infectious diseases. In the present work, we confirmed previous data from our laboratory showing that whole viable bacterial cell treatment with proteases followed by the identification of released peptides by mass spectrometry is the method of choice for the rapid and reliable identification of vaccine candidates in Gram-positive bacteria. When applied to the Group B Streptococcus COH1 strain, 43 surface-associated proteins were identified, including all the protective antigens described in the literature as well as a new protective antigen, the cell wall-anchored protein SAN_1485 belonging to the serine-rich repeat protein family. This strategy overcomes the difficulties so far encountered in the identification of novel vaccine candidates and speeds up the entire vaccine discovery process by reducing the number of recombinant proteins to be tested in the animal model.

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