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Molecular & Cellular Proteomics 8:1823-1831, 2009.
© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Porphyrin-based Photocatalytic Nanolithography

A NEW FABRICATION TOOL FOR PROTEIN ARRAYS^*,

Jane P. Bearinger^,, Gary Stone, Lawrence C. Dugan, Bassem El Dasher, Cheryl Stockton, James W. Conway^¶, Tobias Kuenzler^|| and Jeffrey A. Hubbell^**

From the Lawrence Livermore National Laboratory, Livermore, California 94550,
¶Stanford Nanofabrication Facility, Stanford University, Palo Alto, California 94305,
||Laboratory for Surface Science and Technology, ETH Hönggerberg, HCI F536, CH-8093 Zurich, Switzerland, and
**Institute of Bioengineering and Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, Station 15, CH-1015 Lausanne, Switzerland

Nanoarray fabrication is a multidisciplinary endeavor encompassing materials science, chemical engineering, and biology. We formed nanoarrays via a new technique, porphyrin-based photocatalytic nanolithography. The nanoarrays, with controlled features as small as 200 nm, exhibited regularly ordered patterns and may be appropriate for (a) rapid and parallel proteomics screening of immobilized biomolecules, (b) protein-protein interactions, and/or (c) biophysical and molecular biology studies involving spatially dictated ligand placement. We demonstrated protein immobilization utilizing nanoarrays fabricated via photocatalytic nanolithography on silicon substrates where the immobilized proteins are surrounded by a non-fouling polymer background.

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