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This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M800541-MCP200v1 8/8/1878    most recent Submit a response View responses Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ma, Y. Articles by Qin, H. Search for Related Content PubMed PubMed Citation Articles by Ma, Y. Articles by Qin, H. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 8:1878-1890, 2009.
© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Proteomics Identification of Desmin as a Potential Oncofetal Diagnostic and Prognostic Biomarker in Colorectal Cancer^*,

Yanlei Ma, Jiayuan Peng, Weijie Liu, Peng Zhang, Long Huang, Benbo Gao, Tongyi Shen, Yukun Zhou, Hongqi Chen, Zhaoxin Chu, Ming Zhang and Huanlong Qin^,¶

From the Department of Surgery, The Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China and
Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts 02215

Colorectal cancer (CRC) is the third most common cancer worldwide and has poor prognosis. To identify the oncofetal proteins involved in CRC carcinogenesis, differentially expressed proteins among fetal colorectal tissues, CRC, and the paired tumor-adjacent normal colorectal tissues were investigated by a two-dimensional gel electrophoresis and MALDI-TOF/TOF-based proteomics approach. 42 protein spots were differentially expressed among these tissues, and 22 proteins were identified by MS analysis. Desmin and zinc finger protein 829 were found to be elevated in CRC tissue and fetal colorectal tissue compared with normal colorectal tissue. The elevated expression of desmin in CRC tissue and different developmental stages of fetus colon was confirmed by RT-PCR and Western blot analysis. Immunohistochemical analysis showed that the elevated expression of desmin was correlated with the severity and differentiation of CRC and decreased survival rate of CRC patients. Finally by developing a highly sensitive immunoassay, desmin could be detected in human serum and was significantly elevated in CRC patients compared with healthy volunteers. We propose that desmin be considered a potential oncofetal serum tumor marker for CRC that may have significance in the detection of patients with CRC.

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