HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Free via Author's Choice: AC AC Full Text Full Text (PDF) Supplemental Data AC All Versions of this Article: M900038-MCP200v1 8/9/2119    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via HighWire Google Scholar Articles by DeGrasse, J. A. Articles by Chait, B. T. PubMed PubMed Citation Articles by DeGrasse, J. A. Articles by Chait, B. T. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 8:2119-2130, 2009.
© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Evidence for a Shared Nuclear Pore Complex Architecture That Is Conserved from the Last Common Eukaryotic Ancestor^*,

Jeffrey A. DeGrasse, Kelly N. DuBois, Damien Devos^¶, T. Nicolai Siegel^||, Andrej Sali^**, Mark C. Field, Michael P. Rout and Brian T. Chait^,

From the Laboratories of Mass Spectrometry and Gaseous Ion Chemistry,
||Molecular Parasitology, and
Cellular and Structural Biology, The Rockefeller University, New York, New York 10065,
Department of Pathology, University of Cambridge, The Molteno Building, Tennis Court Road, Cambridge CB2 1QP, United Kingdom,
¶Structural Bioinformatics, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany, and
**Department of Biopharmaceutical Sciences, University of California, San Francisco, California 94158

The nuclear pore complex (NPC) is a macromolecular assembly embedded within the nuclear envelope that mediates bidirectional exchange of material between the nucleus and cytoplasm. Our recent work on the yeast NPC has revealed a simple modularity in its architecture and suggested a common evolutionary origin of the NPC and vesicle coating complexes in a progenitor protocoatomer. However, detailed compositional and structural information is currently only available for vertebrate and yeast NPCs, which are evolutionarily closely related. Hence our understanding of NPC composition in a full evolutionary context is sparse. Moreover despite the ubiquitous nature of the NPC, sequence searches in distant taxa have identified surprisingly few NPC components, suggesting that much of the NPC may not be conserved. Thus, to gain a broad perspective on the origins and evolution of the NPC, we performed proteomics analyses of NPC-containing fractions from a divergent eukaryote (Trypanosoma brucei) and obtained a comprehensive inventory of its nucleoporins. Strikingly trypanosome nucleoporins clearly share with metazoa and yeast their fold type, domain organization, composition, and modularity. Overall these data provide conclusive evidence that the majority of NPC architecture is indeed conserved throughout the Eukaryota and was already established in the last common eukaryotic ancestor. These findings strongly support the hypothesis that NPCs share a common ancestry with vesicle coating complexes and that both were established very early in eukaryotic evolution.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. J. Terry and S. R. Wente Flexible Gates: Dynamic Topologies and Functions for FG Nucleoporins in Nucleocytoplasmic Transport Eukaryot. Cell, December 1, 2009; 8(12): 1814 - 1827. [Abstract] [Full Text] [PDF]