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Submitted on June 10, 2005
Accepted on August 15, 2005
Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455
Corresponding Author: tgriffin{at}umn.edu
Human saliva has great potential for clinical disease diagnostics. Constructing a comprehensive catalogue of saliva proteins using proteomic approaches is a necessary first step to identifying potential protein biomarkers of disease. However, because of the challenge presented in cataloguing saliva proteins with widely varying abundance, new proteomics approaches are needed. To this end, we used a newly developed approach coupling peptide separation using Free Flow Electrophoresis with linear ion trap tandem mass spectrometry to identify proteins in whole human saliva. We identified 437 proteins with high confidence (false positive rate below 1%), producing the largest catalogue of proteins from a single saliva sample to date and providing new information on the composition and potential diagnostic utility of this fluid. The statistically validated, transparently presented, and annotated dataset provides a model for presenting large-scale proteomic data of this type, which should facilitate better dissemination and easier comparisons of proteomic datasets from future studies in saliva.
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