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Submitted on April 26, 2004
The Institute for Systems Biology, Seattle, WA 98103-8904
Corresponding Author: qtian{at}systemsbiology.org
Using DNA microarrays together with quantitative proteomic techniques [isotope-coded affinity tags (ICAT) reagents, 2-D differential in-gel electrophoresis (2-D DIGE), and mass spectrometry], we evaluated the correlation of mRNA and protein levels in two hematopoietic cell lines representing distinct stages of myeloid differentiation, as well as in the livers of mice treated for different periods of time with three different PPAR (peroxisome proliferative activated receptor) agonists. We observe that the differential expression of mRNA (up or down) can capture at most 40% of the variation of protein expression. Although the overall pattern of protein expression is similar to that of mRNA expression, the incongruent expression between mRNAs and proteins emphasize the importance of posttranscriptional regulatory mechanisms in cellular development or perturbation that can be unveiled only through integrated analyses of both proteins and mRNAs.
Revised on June 23, 2004
Accepted on July 6, 2004
Integrated genomic and proteomic analyses of gene expression in mammalian cells
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