MCP Waters-The Science of What's Possible
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on November 1, 2005.
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M500062-MCP200v1
4/11/1710    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Glossary
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Birner-Gruenberger, R.
Right arrow Articles by Hermetter, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Birner-Gruenberger, R.
Right arrow Articles by Hermetter, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on March 4, 2005
Revised on July 5, 2005
Accepted on July 26, 2005

The lipolytic proteome of mouse adipose tissue

Ruth Birner-Gruenberger, Heidrun Susani-Etzerodt, Markus Waldhuber, Gernot Riesenhuber, Hannes Schmidinger, Gerald Rechberger, Manfred Kollroser, Juliane G. Strauss, Achim Lass, Robert Zimmermann, Guenter Haemmerle, Rudolf Zechner, and Albin Hermetter

Biochemistry Dept., Graz University of Technology, Graz A-8010

Corresponding Author: albin.hermetter{at}tugraz.at

Hydrolysis of triacylglycerols and cholesteryl esters is a key event in energy homeostasis of animals. However, many lipolytic activities still await their molecular identification. Here we report on a novel tool for concomitant analysis of lipases in complex proteomes. Fluorescent activity tags mimicking lipid substrates were used to label the proteome of mouse adipose tissue. Analysis by 2D-gel electrophoresis and LC-MS/MS led to the identification of all known intracellular lipases, as well as a number of novel candidates. One of them was recently shown to be involved in triacylglycerol mobilization in adipocytes and therefore named adipose triglyceride lipase. Functional characterization of expressed enzymes demonstrated that lipolytic and esterolytic activities could be well discriminated. Thus our results show the first map of the lipolytic proteome of mouse adipose tissue and demonstrate the general applicability of our method for rapid profiling and identification of lipolytic activities in complex biological samples.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Endocr. Rev.Home page
M. Prentki and S. R. M. Madiraju
Glycerolipid Metabolism and Signaling in Health and Disease
Endocr. Rev., October 1, 2008; 29(6): 647 - 676.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
G. R. Steinberg, B. E. Kemp, and M. J. Watt
Adipocyte triglyceride lipase expression in human obesity
Am J Physiol Endocrinol Metab, October 1, 2007; 293(4): E958 - E964.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Wei, W. Gao, and R. Lehner
Attenuation of Adipocyte Triacylglycerol Hydrolase Activity Decreases Basal Fatty Acid Efflux
J. Biol. Chem., March 16, 2007; 282(11): 8027 - 8035.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Robidoux, N. Kumar, K. W. Daniel, F. Moukdar, M. Cyr, A. V. Medvedev, and S. Collins
Maximal beta3-Adrenergic Regulation of Lipolysis Involves Src and Epidermal Growth Factor Receptor-dependent ERK1/2 Activation
J. Biol. Chem., December 8, 2006; 281(49): 37794 - 37802.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.