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Submitted on April 26, 2005
Revised on June 14, 2005
Accepted on June 18, 2005

Accelerated discovery of novel protein function in cultured human cells

Emily Hodges, Jenny Redelius, Weilin Wu, and Christer Höög

Center for Genomics and Bioinformatics, Karolinska Institutet, Stockholm S-171 77

Corresponding Author: christer.hoog{at}cmb.ki.se

Experimental approaches which enable direct investigation of human protein function are necessary for comprehensive annotation of the human proteome. We introduce a cell-based platform for rapid and unbiased functional annotation of under-characterized human proteins. Utilizing a library of antibody biomarkers, the full-length proteins are investigated by tracking phenotypic changes caused by overexpression in human cell lines. We combine reverse transfection and immunodetection by fluorescence microscopy to facilitate this procedure at highresolution. Demonstrating the advantage of this approach, new annotations are provided for two novel proteins, (1) a membrane-bound O-acyltransferase protein (C3F) that, when overexpressed, disrupts Golgi and endosome integrity due likely to an ER-Golgi transport block, and (2) a tumor marker (BC-2) that prompts a redistribution of a transcriptional silencing protein (BMI1) and a MAPKinase mediator (Rac1) to distinct nuclear regions that undergo chromatin compaction. Our strategy is an immediate application for directly addressing those proteins whose molecular function remains unknown.


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