MCP Agilent Technologies
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on October 1, 2007.
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M700057-MCP200v1
6/10/1771    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Glossary
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Everley, P. A.
Right arrow Articles by Gygi, S. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Everley, P. A.
Right arrow Articles by Gygi, S. P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on February 9, 2007
Revised on July 10, 2007
Accepted on July 11, 2007

Assessing enzyme activities using stable isotope labeling and mass spectrometry

Patrick A. Everley, Carlos A. Gartner, Wilhelm Haas, Alan Saghatelian, Joshua E. Elias, Benjamin F. Cravatt, Bruce R. Zetter, and Steven P. Gygi

Cell Biology, Harvard Medical School, Boston, MA 02115

Corresponding Author: steven_gygi{at}hms.harvard.edu

Activity-based protein profiling has emerged as a valuable technology for labeling, enriching and assessing protein activities from complex mixtures. This is primarily accomplished via a two step identification and quantification process. Here we show a highly quantitative and streamlined method, termed Catch-and-release Activity Profiling of Enzymes (CAPE), which reduces this procedure to a single step. Furthermore, the CAPE approach has the ability to detect small quantitative changes that may have been missed by alternative mass spectrometry-based techniques.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
L. C. J. Gillet, K. Namoto, A. Ruchti, S. Hoving, D. Boesch, B. Inverardi, D. Mueller, M. Coulot, P. Schindler, P. Schweigler, et al.
In-cell Selectivity Profiling of Serine Protease Inhibitors by Activity-based Proteomics
Mol. Cell. Proteomics, July 1, 2008; 7(7): 1241 - 1253.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2007 by the American Society for Biochemistry and Molecular Biology.