| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on September 16, 2002
Cell Biology, The Scripps Research Institute, La Jolla, CA 92037
Corresponding Author: cravatt{at}scripps.edu
With complete genome sequences now available for several prokaryotic and eukaryotic organisms, biological researchers are charged with the task of assigning molecular and cellular functions to thousands of predicted gene products. To address this problem, the field of proteomics seeks to develop and apply methods for the global analysis of protein expression and protein function. Here, we review a promising new class of proteomics strategies that employs synthetic chemistry to create tools and assays for the characterization of protein samples of high complexity. These approaches include the development of chemical affinity tags to measure the relative expression level and post-translational modification state of proteins in cell and tissue proteomes. Additionally, we will discuss the emerging field of activity-based protein profiling, which aims to synthesize and apply small molecule probes that monitor dynamics in protein function in complex proteomes.
Revised on October 3, 2002
Accepted on October 3, 2002
Chemical strategies for functional proteomics
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. J. Ratcliffe, T. Yi, and S. S. Khandekar Synthesis and Characterization of 5'-p-fluorosulfonylbenzoyl-2' (or 3')-(biotinyl)adenosine as an Activity-Based Probe for Protein Kinases J Biomol Screen, February 1, 2007; 12(1): 126 - 132. [Abstract] [PDF]
|
|
|
|
 |
|
 R. Birner-Gruenberger, H. Susani-Etzerodt, M. Waldhuber, G. Riesenhuber, H. Schmidinger, G. Rechberger, M. Kollroser, J. G. Strauss, A. Lass, R. Zimmermann, et al. The Lipolytic Proteome of Mouse Adipose Tissue Mol. Cell. Proteomics, November 1, 2005; 4(11): 1710 - 1717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Khandekar, B. Feng, T. Yi, S. Chen, N. Laping, and N. Bramson A Liquid Chromatography/Mass Spectrometry-Based Method for the Selection of ATP Competitive Kinase Inhibitors J Biomol Screen, August 1, 2005; 10(5): 447 - 455. [Abstract] [PDF]
|
|
|
|
 |
|
 S. C. Peck Update on Proteomics in Arabidopsis. Where Do We Go From Here? Plant Physiology, June 1, 2005; 138(2): 591 - 599. [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Okerberg, J. Wu, B. Zhang, B. Samii, K. Blackford, D. T. Winn, K. R. Shreder, J. J. Burbaum, and M. P. Patricelli High-resolution functional proteomics by active-site peptide profiling PNAS, April 5, 2005; 102(14): 4996 - 5001. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. E. Carver, B. Bordeau, M. D. Cummings, E. C. Petrella, M. J. Pucci, L. E. Zawadzke, B. A. Dougherty, J. A. Tredup, J. W. Bryson, J. Yanchunas Jr., et al. Decrypting the Biochemical Function of an Essential Gene from Streptococcus pneumoniae Using ThermoFluor(R) Technology J. Biol. Chem., March 25, 2005; 280(12): 11704 - 11712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Saghatelian, N. Jessani, A. Joseph, M. Humphrey, and B. F. Cravatt Activity-based probes for the proteomic profiling of metalloproteases PNAS, July 6, 2004; 101(27): 10000 - 10005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. M. Cristea, S. J. Gaskell, and A. D. Whetton Proteomics techniques and their application to hematology Blood, May 15, 2004; 103(10): 3624 - 3634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Machida, B. J. Mayer, and P. Nollau Profiling the Global Tyrosine Phosphorylation State Mol. Cell. Proteomics, April 1, 2003; 2(4): 215 - 233. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Journal of Lipid Research | ASBMB Today |
