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Submitted on February 27, 2004
Revised on February 28, 2004
Accepted on February 28, 2004

Mass spectrometry as a diagnostic and a cancer biomarker discovery tool: Opportunities and potential limitations

Eleftherios P. Diamandis

Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario M5G 1X5

Corresponding Author: ediamandis{at}mtsinai.on.ca

Serum proteomic profiling, by using surfaced-enhanced laser desorbtion ionization-time-of-flight mass spectrometry (SELDI-TOF), is one of the most promising new approaches for cancer diagnostics. Exceptional sensitivities and specificities have been reported for some cancer types such as prostate, ovarian, breast and bladder cancers. These sensitivities/specificities are far superior to those obtained by using classical cancer biomarkers. In this review, I concentrate more on questions which cast doubt on the results reported and propose experiments to investigate these questions in detail, before the technique is used at the clinic. It is clear that the method needs to be externally and thoroughly validated before clinical implementation is warranted.


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