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A more recent version of this article appeared on August 1, 2006.
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Submitted on March 13, 2006
Revised on May 11, 2006
Accepted on May 30, 2006

Proteomics-based expression library screening (PELS) - a novel method for rapidly defining microbial immunoproteomes

Indira T. Kudva, Bryan Krastins, Haiqing Sheng, Robert W. Griffin, David A. Sarracino, Phillip I. Tarr, Carolyn J. Hovde, Stephen B. Calderwood, and Manohar John

Division of Infectious Diseases, Dept. of Medicine, Massachusetts General Hospital, Boston, MA 02114

Corresponding Author: mjohn1{at}partners.org

Current methodologies for global identification of microbial proteins that elicit host humoral immune responses have several limitations, and are not ideally suited for use in the post-genomic era. Here, we describe a novel application of proteomics, Proteomics-based Expression Library Screening (PELS), to rapidly define microbial immunoproteomes. PELS is broadly applicable to any cultivable, sequenced pathogen eliciting host antibody responses, and hence is ideal for rapidly mining microbial proteomes for targets with diagnostic, prophylactic and therapeutic potential. In this report, we demonstrate “proof-of-principle” by identifying 207 proteins of the Escherichia coli O157:H7 immunome in bovine reservoirs in only three weeks.


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This article has been cited by other articles:


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[Abstract] [Full Text] [PDF]


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