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Molecular & Cellular Proteomics 6:1287-1288, 2007.
© 2007 by The American Society for Biochemistry and Molecular Biology, Inc.
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From the Department of Anatomy and Cell Biology, McGill University, Montreal H3A 2B2, Canada and ¶ Fred Hutchison Cancer Research Center, Seattle, Washington 98109
The HUPO Initiative Committee organized its first meeting in Barbados in January 2007 in conjunction with the Third International Barbados Proteomics Conference.
Held January 5–11, 2007, and organized by Laura Beretta (chair, Human Liver Proteome Project) and John Bergeron (chair, HUPO Initiatives), it was enthusiastically attended by HUPO Initiatives chairs, co-chairs, and collaborators as well as representatives from research projects involved in a mouse model of human disease initiative (chaired by Bergeron) and editors from Molecular & Cellular Proteomics (Ralph Bradshaw) and Nature Methods (Veronique Kiermer).
The week-long meeting saw vibrant discussions from the various initiatives chairs and also resulted in the drafting of a document on the future of HUPO Initiatives entitled "The Barbados Principles."
The choice of McGill University's Bellairs Research Institute in Holetown, Barbados, (Canada's only teaching and research facility in the tropics) as a location provided all participants with the opportunity to have breakfast together and then attend presentations in an idyllic setting conducive to brainstorming. Although afternoons were left free, they were mostly used for discussion stemming from the morning presentations. Later on in the day, the presentations resumed for the best part of the evening and were then followed by late evening activities, which also included further discussion on the day's activities.
The first day consisted of an overview by Bergeron of the objectives and expectations of this conference along with detailed presentations on organellar proteomics as applied to the liver. The next day, Peter Liu (Toronto, co-chair of the HUPO Cardiovascular Initiative) presented an exciting integration of proteomics and genomics analysis of the cardiovascular system. Michel Desjardins (Montreal) described a paradigm shift occurring through proteomics for our understanding of infectious diseases in mouse model systems, and then Gil Omenn (Ann Arbor) exhaustively described the heroic effort of the Human Plasma Proteome Project and the challenges encountered to put this effort together for its pilot phase. This ended with an explanation of where the project is currently heading under Omenn's guardianship along with co-chairs Young-Ki Paik and Ruedi Aebersold. Eric Deutsch (Seattle) from the Institute for Systems Biology rounded up the evening with a presentation on the PeptideAtlas project and its immense value to the HUPO community.
On day three, Paul Jolicoeur (Montreal) presented an exciting elaboration of a mouse model of AIDS ripe for detailed characterization by proteomics. Mike Snyder (New Haven, co-chair of the Human Antibody Initiative and President of USHUPO) presented an overview of the human antibody initiative as well as protein chip-based proteomics. Naoyuki Taniguchi (Osaka) gave a summary of the Human Disease Glycomics/Proteome Initiative and its first characterization of standards throughout the glycomics community. He also presented the importance of these modifications for liver disease biomarker discovery. Christoph Borchers (Victoria, British Columbia) impressed the audience with his description of new proteomics-based methods for clinical diagnostic biomarker discovery and structural proteomics. Here the applications of leading edge technologies for quantitative proteomics and exquisite sensitivity of protein detection were highlighted.
The evening session was lengthy and started with Veronique Kiermer (of Nature Methods) and Beretta (Seattle) gathering a consensus to define priorities and objectives of what was to be achieved at this conference. These included a formal indication of what proteomics has accomplished to date; Kiermer also addressed the expectations of the research community with respect to funding of international activities of proteomics (i.e. how funding agencies from different countries could address research needs in a coordinated fashion with respect to proteomics). Most importantly, the attendees were challenged to come up with a simple mission statement to guide the development of a single HUPO project. This ended with a detailed, comprehensive presentation from Deutsch, here representing the Proteomics Standards Initiative (PSI) and its coordination with HUPO activities, and then with Beretta summarizing the exciting work on the Human Liver Proteome Project and liver biomarkers for disease.
Day four was a heavy day. It opened early with Bradshaw giving a detailed presentation on challenges facing the Human Proteome Organization. Bradshaw also discussed the scientific aspects of how MCP has attempted to coordinate with the PSI in establishing a standardized format to enable the community to assure they have met criteria for peptide characterization. This was followed by talks by Tom Beardslee from Invitrogen and Alex Bell (Montreal), who presented the progress on the HUPO Test Samples. These test samples have been sent to 24 labs selected by the HUPO Test Samples Committee. The final report in which the 24 labs have participated will define a path from initial analysis of the test sample proteins to a comprehensive analysis of the raw data submitted from each laboratory. This will be followed by a final re-assessment of new test samples to assure a 100% success rate for any HUPO member. Ultimately, the goal of this effort is to use education and training to get 100% successful analysis. The following session was a presentation by Bell, who characterized the proteins of the liver detoxification machinery. The morning then ended with a presentation from David Juncker (Montreal) on the application of new leading edge technologies involving microfluidics to effect large scale ELISAs to conduct proteomics. The afternoon session was started with Beretta, Omenn, and Snyder, who put together the mission and governing structure for current HUPO initiatives. This has been approved by HUPO and is currently on the HUPO Web site at www.hupo.org.
Later on, Kiermer gathered together everyone's proposals to draft a position paper on the single HUPO project, which was assigned to Snyder and Bergeron. Bradshaw and Bergeron then were assigned to put together a short review of what proteomics has accomplished to date. In lieu of the evening session, the group went to the Barbados Jazz Festival held at St. Phillip for a change of pace and a welcome break.
The final day (January 10) began with an exciting presentation by Tommy Nilsson (Gotenburg, Sweden), who heads an effort using mouse models of obesity in an organellar proteomics approach to characterize proteins in the adipocyte causally implicated in genetics models of obesity. Tadashi Yamamoto (Niigata, Japan) provided an exciting overview of the kidney initiative in which detailed analysis of human kidney and urine were presented as well as a coordination of proteins characterization through this initiative with the same proteins characterized by the Human Protein Atlas Initiative. Ken Standing (Winnipeg) provided a tour de force presentation in which advanced mass spectrometric methods were used to characterize plant pathogenic viruses, several from unsequenced genomes, and developed therapeutic treatment simply from the proteomics itself.
The final scientific presentation was from Bergeron, who summarized the initiative on mouse models of human disease and then concluded by giving a summary perspective of the Barbados conference.
Key deliverables from this meeting were a set of Barbados principles. These included a new governing structure for HUPO initiatives as well as a path forward on the make up of the HUPO project and a recapitulation of what proteomics has accomplished to date.
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FOOTNOTES |
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Chair of HUPO Initiatives and co-chair of the 3rd International Proteomics conference, Barbados. To whom correspondence should be addressed. E-mail: john.bergeron{at}mcgill.ca
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