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Originally published In Press as doi:10.1074/mcp.M400048-MCP200 on May 6, 2004.
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Molecular & Cellular Proteomics 3:809-819, 2004.
© 2004 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Biochemical Characterization of Protein Complexes from the Helicobacter pylori Protein Interaction Map

Strategies for Complex Formation and Evidence for Novel Interactions Within Type IV Secretion Systems*

Laurent Terradot{ddagger},§, Nathan Durnell{ddagger}, Min Li{ddagger}, Ming Li{ddagger}, Jeremiah Ory{ddagger}, Agnes Labigne, Pierre Legrain||,**, Frederic Colland|| and Gabriel Waksman{ddagger},§,{ddagger}{ddagger},§§

From the {ddagger} Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, 660 South Euclid Avenue, Campus Box 8231, St. Louis, MO 63110; § Institute of Structural Molecular Biology, Birkbeck College and University College London, University of London, Malet Street, Bloomsbury, London WC1E 7HX, United Kingdom; Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, 75724 Paris Cedex 15, France; || Hybrigenics, 3-5 Impasse Reille, 75014 Paris, France; and {ddagger}{ddagger} University College London, Department of Biochemistry and Molecular Biology, Gower Street, London WC1E 6BT, United Kingdom

We have investigated a large set of interactions from the Helicobacter pylori protein interaction map previously identified by high-throughput yeast two-hybrid (htY2H)-based methods. This study had two aims: i) to validate htY2H as a source of protein-protein interaction complexes for high-throughput biochemical and structural studies of the H. pylori interactome; and ii) to validate biochemically interactions shown by htY2H to involve components of the H. pylori type IV secretion systems. Thus, 17 interactions involving 31 proteins and protein fragments were studied, and a general strategy was designed to produce protein-interacting partners for biochemical and structural characterization. We show that htY2H is a valid source of protein-protein complexes for high-throughput proteome-scale characterization of the H. pylori interactome, because 76% of the interactions tested were confirmed biochemically. Of the interactions involving type IV secretion proteins, three could be confirmed. One interaction is between two components of the type IV secretion apparatus, ComB10 and ComB4, which are VirB10 and VirB4 homologs, respectively. Another interaction is between a type IV component (HP0525, a VirB11 homolog) and a non-type IV secretion protein (HP01451), indicating that proteins other than the core VirB (1-11)-VirD4 proteins may play a role in type IV secretion. Finally, a third interaction was biochemically confirmed between CagA, a virulence factor secreted by the type IV secretion system encoded by the Cag pathogenicity island, and a non-type IV secretion protein, HP0496.

§§ To whom correspondence should be addressed: Birkbeck College, University of London, Department of Crystallography, Malet Street, Bloomsbury, London WC1E 7HX, United Kingdom. Tel.: 44-(0) 20-7631-6833; Fax: 44-(0) 20-7631-6803; E-mail: g.waksman{at}mail.cryst.bbk.ac.uk


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