MCP Agilent Technologies
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.D500008-MCP200 on August 15, 2005.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
D500008-MCP200v1
4/11/1826    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Glossary
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xie, H.
Right arrow Articles by Griffin, T. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xie, H.
Right arrow Articles by Griffin, T. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 4:1826-1830, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

Dataset

A Catalogue of Human Saliva Proteins Identified by Free Flow Electrophoresis-based Peptide Separation and Tandem Mass Spectrometry*,S

Hongwei Xie{ddagger}, Nelson L. Rhodus§, Robert J. Griffin, John V. Carlis|| and Timothy J. Griffin{ddagger},**

From the Departments of {ddagger} Biochemistry, Molecular Biology, and Biophysics, § Oral Medicine, Diagnosis, and Radiology, School of Dentistry, Therapeutic Radiology-Radiation Oncology, and || Computer Science and Engineering, University of Minnesota, Minneapolis, Minnesota 55455

Human saliva has great potential for clinical disease diagnostics. Constructing a comprehensive catalogue of saliva proteins using proteomic approaches is a necessary first step to identifying potential protein biomarkers of disease. However, because of the challenge presented in cataloguing saliva proteins with widely varying abundance, new proteomic approaches are needed. To this end, we used a newly developed approach coupling peptide separation using free flow electrophoresis with linear ion trap tandem mass spectrometry to identify proteins in whole human saliva. We identified 437 proteins with high confidence (false positive rate below 1%), producing the largest catalogue of proteins from a single saliva sample to date and providing new information on the composition and potential diagnostic utility of this fluid. The statistically validated, transparently presented, and annotated dataset provides a model for presenting large scale proteomic data of this type, which should facilitate better dissemination and easier comparisons of proteomic datasets from future studies in saliva.

** To whom correspondence should be addressed: Dept. of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, 6-155 Jackson Hall, 321 Church St. S.E., Minneapolis, MN 55455. Tel.: 612-624-5249; Fax: 612-624-0432; E-mail: tgriffin{at}umn.edu


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
H. Xie, G. Onsongo, J. Popko, E. P. de Jong, J. Cao, J. V. Carlis, R. J. Griffin, N. L. Rhodus, and T. J. Griffin
Proteomics Analysis of Cells in Whole Saliva from Oral Cancer Patients via Value-added Three-dimensional Peptide Fractionation and Tandem Mass Spectrometry
Mol. Cell. Proteomics, March 1, 2008; 7(3): 486 - 498.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
P. A. Grimsrud, M. J. Picklo Sr., T. J. Griffin, and D. A. Bernlohr
Carbonylation of Adipose Proteins in Obesity and Insulin Resistance: Identification of Adipocyte Fatty Acid-binding Protein as a Cellular Target of 4-Hydroxynonenal
Mol. Cell. Proteomics, April 1, 2007; 6(4): 624 - 637.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
Y. Andersch-Bjorkman, K. A. Thomsson, J. M. Holmen Larsson, E. Ekerhovd, and G. C. Hansson
Large Scale Identification of Proteins, Mucins, and Their O-Glycosylation in the Endocervical Mucus during the Menstrual Cycle
Mol. Cell. Proteomics, April 1, 2007; 6(4): 708 - 716.
[Abstract] [Full Text] [PDF]

Home page
 Occup. Environ. Med.Home page
D. Soo-Quee Koh and G. Choon-Huat Koh
The use of salivary biomarkers in occupational and environmental medicine
Occup. Environ. Med., March 1, 2007; 64(3): 202 - 210.
[Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
W.-J. Qian, J. M. Jacobs, T. Liu, D. G. Camp II, and R. D. Smith
Advances and Challenges in Liquid Chromatography-Mass Spectrometry-based Proteomics Profiling for Clinical Applications
Mol. Cell. Proteomics, October 1, 2006; 5(10): 1727 - 1744.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.