HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M500405-MCP200v1 5/7/1261    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Van Hoof, D. Articles by Krijgsveld, J. Search for Related Content PubMed PubMed Citation Articles by Van Hoof, D. Articles by Krijgsveld, J. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 5:1261-1273, 2006.
© 2006 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

A Quest for Human and Mouse Embryonic Stem Cell-specific Proteins ^*,S

Dennis Van Hoof^,, Robert Passier^,¶, Dorien Ward-Van Oostwaard^,¶, Martijn W. H. Pinkse, Albert J. R. Heck, Christine L. Mummery^,¶,|| and Jeroen Krijgsveld^,**

From the Hubrecht Laboratory, Netherlands Institute of Developmental Biology, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands, Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands, and ^¶ Interuniversity Cardiology Institute of the Netherlands and Heart Lung Institute, University Medical Centre Utrecht, 3584 CX Utrecht, The Netherlands

Embryonic stem cells (ESCs) are of immense interest as they can proliferate indefinitely in vitro and give rise to any adult cell type, serving as a potentially unlimited source for tissue replacement in regenerative medicine. Extensive analyses of numerous human and mouse ESC lines have shown generic similarities and differences at both the transcriptional and functional level. However, comprehensive proteome analyses are missing or are restricted to mouse ESCs. Here we have used an extensive proteomic approach to search for ESC-specific proteins by analyzing the differential protein expression profiles of human and mouse ESCs and their differentiated derivatives. The data sets comprise 1,775 non-redundant proteins identified in human ESCs, 1,532 in differentiated human ESCs, 1,871 in mouse ESCs, and 1,552 in differentiated mouse ESCs with a false positive rate of <0.2%. Comparison of the data sets distinguished 191 proteins exclusively identified in both human and mouse ESCs but not in their differentiated derivatives. Besides well known ESC benchmarks, this subset included many uncharacterized proteins, some of which may be novel ESC-specific markers. To complement the mass spectrometric approach, differential expression of a selection of these proteins was confirmed by Western blotting, immunofluorescence confocal microscopy, and fluorescence-activated cell sorting. Additionally two other independently isolated and cultured human ESC lines as well as their differentiated derivatives were monitored for differential expression of selected proteins. Some of these proteins were identified exclusively in ESCs of all three human lines and may thus serve as generic ESC markers. Our wide scale proteomic approach enabled us to screen thousands of proteins rapidly and select putative ESC-associated proteins for further analysis. Validation by three independent conventional protein analysis techniques shows that our methodology is robust, provides an excellent tool to characterize ESCs at the protein level, and may disclose novel ESC-specific benchmarks.

^** To whom correspondence may be addressed. Tel.: 31-30-253-9974; Fax.: 31-30-251-6464; E-mail: j.krijgsveld{at}chem.uu.nl

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. Van Hoof, S. R. Braam, W. Dormeyer, D. Ward-van Oostwaard, A. J.R. Heck, J. Krijgsveld, and C. L. Mummery Feeder-Free Monolayer Cultures of Human Embryonic Stem Cells Express an Epithelial Plasma Membrane Protein Profile Stem Cells, November 1, 2008; 26(11): 2777 - 2781. [Abstract] [Full Text] [PDF]

				J. Graumann, N. C. Hubner, J. B. Kim, K. Ko, M. Moser, C. Kumar, J. Cox, H. Scholer, and M. Mann Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Proteome Quantitation of Mouse Embryonic Stem Cells to a Depth of 5,111 Proteins Mol. Cell. Proteomics, April 1, 2008; 7(4): 672 - 683. [Abstract] [Full Text] [PDF]

				A. K. Yocum, T. E. Gratsch, N. Leff, J. R. Strahler, C. L. Hunter, A. K. Walker, G. Michailidis, G. S. Omenn, K. S. O'Shea, and P. C. Andrews Coupled Global and Targeted Proteomics of Human Embryonic Stem Cells during Induced Differentiation Mol. Cell. Proteomics, April 1, 2008; 7(4): 750 - 767. [Abstract] [Full Text] [PDF]

				H. Baharvand, A. Fathi, D. van Hoof, and G. H. Salekdeh Concise Review: Trends in Stem Cell Proteomics Stem Cells, August 1, 2007; 25(8): 1888 - 1903. [Abstract] [Full Text] [PDF]