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Molecular & Cellular Proteomics 5:1514-1519, 2006.
© 2006 by The American Society for Biochemistry and Molecular Biology, Inc.

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Technology

Proteomics-based Expression Library Screening (PELS)

A Novel Method for Rapidly Defining Microbial Immunoproteomes^*,S

Indira T. Kudva^,,¶, Bryan Krastins^¶,||, Haiqing Sheng^**, Robert W. Griffin, David A. Sarracino^||, Phillip I. Tarr, Carolyn J. Hovde^**,, Stephen B. Calderwood^,,¶¶ and Manohar John^,,||||

From the Division of Infectious Diseases, Massachusetts General Hospital and Departments of Medicine and ^¶¶ Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02114, ^|| Harvard Partners Center For Genetics and Genomics, Cambridge, Massachusetts 02139, ^** Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844, and Departments of Pediatrics and Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110

Current methodologies for global identification of microbial proteins that elicit host humoral immune responses have several limitations and are not ideally suited for use in the postgenomic era. Here we describe a novel application of proteomics, proteomics-based expression library screening, to rapidly define microbial immunoproteomes. Proteomics-based expression library screening is broadly applicable to any cultivable, sequenced pathogen eliciting host antibody responses and hence is ideal for rapidly mining microbial proteomes for targets with diagnostic, prophylactic, and therapeutic potential. In this report, we demonstrate "proof-of-principle" by identifying 207 proteins of the Escherichia coli O157:H7 immunome in bovine reservoirs in only 3 weeks.

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