HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M600428-MCP200v1 6/10/1671    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Deniel, N. Articles by Thébault, S. Search for Related Content PubMed PubMed Citation Articles by Deniel, N. Articles by Thébault, S. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 6:1671-1679, 2007.
© 2007 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Glutamine Regulates the Human Epithelial Intestinal HCT-8 Cell Proteome under Apoptotic Conditions^*,S

Nicolas Deniel^,, Rachel Marion-Letellier, Roland Charlionet^¶, François Tron^¶, Jérôme Leprince^||, Hubert Vaudry^||, Philippe Ducrotté, Pierre Déchelotte and Sandrine Thébault^,,**

From the Groupe Aden EA3234 and ^¶ INSERM UR519, Université de Rouen, IFRMP23, 22 boulevard Gambetta, 76183 Rouen cedex 1, France and ^|| INSERM UR413, Université de Rouen, IFRMP23, place Emile Blondel, 76821 Mont-Saint-Aignan, France

Glutamine plays a key role in the metabolism of rapidly dividing cells, including enterocytes and lymphocytes, which may contribute to its beneficial clinical effects. Gut mucosal homeostasis is achieved through a balance between cell proliferation and apoptosis. In T cells, glutamine up-regulates antiapoptotic proteins and down-regulates proapoptotic proteins. In gut mucosa, glutamine prevents apoptosis in rat epithelial cell lines, whereas glutamine starvation induces apoptosis through caspase activation. Finally glutamine specifically prevents tumor necrosis factor--related apoptosis in the human intestinal cell line HT-29. Comparative functional proteomics enables the characterization of each differentially expressed protein in intestinal cells in response to modifications of nutritional environment. The influence of glutamine on intestinal proteome expression in apoptotic conditions has not been studied and evaluated. This comparative proteomics study was performed in the human epithelial intestinal cell line HCT-8 under experimental apoptotic conditions to investigate the influence of glutamine on protein expression during apoptosis. The pharmaconutritional effects of glutamine were determined under 2 mM (physiological concentration) and 10 mM (pharmaconutritional concentration) conditions. About 1,800 protein spots were revealed in both conditions. Comparative assessments indicated that 28 proteins were differentially expressed significantly (i.e. at least 2-fold modulated and Student's t test with p 0.05) in response to an increase of glutamine concentration in the culture medium. Twenty-four proteins were identified by mass spectrometry and associated databases. From these proteins, 34% are involved in cell cycle and apoptosis mechanisms, 17% are involved in signal transduction, and 13% are involved in cytoskeleton organization. These data were integrated in a proposed schema of the interactome under apoptotic conditions. In conclusion, this study provides the first holistic picture of proteome modulation by glutamine in a human enterocytic cell line under apoptotic conditions and supports further evaluation of nutritional modulation of human intestinal proteome in various pathological conditions where apoptosis may be involved.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Coeffier, S. Claeyssens, S. Lecleire, J. Leblond, A. Coquard, C. Bole-Feysot, A. Lavoinne, P. Ducrotte, and P. Dechelotte Combined enteral infusion of glutamine, carbohydrates, and antioxidants modulates gut protein metabolism in humans Am. J. Clinical Nutrition, November 1, 2008; 88(5): 1284 - 1290. [Abstract] [Full Text] [PDF]

				S.-Z. Zhang, H.-Q. Xie, Y. Xu, X.-Q. Li, R.-Q. Wei, W. Zhi, L. Deng, L. Qiu, and Z.-M. Yang Regulation of Cell Proliferation by Fast Myosin Light Chain 1 in Myoblasts Derived from Extraocular Muscle, Diaphragm and Gastrocnemius Experimental Biology and Medicine, November 1, 2008; 233(11): 1374 - 1384. [Abstract] [Full Text] [PDF]