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Originally published In Press as doi:10.1074/mcp.M700158-MCP200 on July 20, 2007.
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Molecular & Cellular Proteomics 6:1818-1823, 2007.
© 2007 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Proteomics Identification of Proteins in Human Cortex Using Multidimensional Separations and MALDI Tandem Mass Spectrometer*,S

Sheng Pan{ddagger}, Min Shi{ddagger}, Jinghua Jin{ddagger}, Roger L. Albin§, Andy Lieberman§, Marla Gearing, Biaoyang Lin||, Catherine Pan{ddagger}, Xiaowei Yan||, Daniel T. Kashima{ddagger} and Jing Zhang{ddagger},**

From the {ddagger} Department of Pathology, University of Washington School of Medicine, Seattle, Washington, 98104, § Ann Arbor Veterans Affairs Medical Center Geriatric Research Education and Clinical Center and Departments of Neurology and Pathology, University of Michigan, Ann Arbor, Michigan 48109, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30322, and || Institute for Systems Biology, Seattle, Washington 98103

It is essential to characterize the proteome of various regions of human brain because most, if not all, neurodegenerative diseases are region-specific. Here we report an in-depth proteomics identification of proteins extracted from the frontal cortex, a region playing a critical role in cognitive function. The integrated proteomics analytical flow consisted of biochemical fractionation, strong cation exchange chromatography, reverse phase liquid chromatography, and MALDI-TOF/TOF mass spectrometric analysis. In total, 812 proteins were confidently identified with two or more peptides. These proteins demonstrated diverse isoelectric points and molecular weights and are involved in several molecular functions, including protein binding, catalytic activity, transport, structure, and signal transduction. A number of proteins known to be associated with neurodegenerative diseases were also identified. Detailed characterization of these proteins will supply the necessary information to appropriately interpret proteins associated with aging and/or age-related neurodegenerative diseases. Finally 140 proteins found in the cortical proteome were present in the proteome of cerebrospinal fluid, providing tissue-specific candidates for biomarker discovery in body fluid.

** To whom correspondence should be addressed: Div. of Neuropathology, Harborview Medical Center, University of Washington School of Medicine, Box 359635, 325 9th Ave., Seattle, WA 98104-2499. Tel.: 206-341-5245; E-mail: zhangj{at}u.washington.edu


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Copyright © 2007 by the American Society for Biochemistry and Molecular Biology.