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Molecular & Cellular Proteomics 6:1868-1884, 2007.
© 2007 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Comparative Proteomics Analysis of Differentially Expressed Proteins in Chickpea Extracellular Matrix during Dehydration Stress^*,S

Deepti Bhushan, Aarti Pandey, Mani Kant Choudhary, Asis Datta, Subhra Chakraborty and Niranjan Chakraborty

From the National Centre for Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India

Water deficit or dehydration is the most crucial environmental factor that limits crop productivity and influences geographical distribution of many crop plants. It is suggested that dehydration-responsive changes in expression of proteins may lead to cellular adaptation against water deficit conditions. Most of the earlier understanding of dehydration-responsive cellular adaptation has evolved from transcriptome analyses. By contrast, comparative analysis of dehydration-responsive proteins, particularly proteins in the subcellular fraction, is limiting. In plants, cell wall or extracellular matrix (ECM) serves as the repository for most of the components of the cell signaling process and acts as a frontline defense. Thus, we have initiated a proteomics approach to identify dehydration-responsive ECM proteins in a food legume, chickpea. Several commercial chickpea varieties were screened for the status of dehydration tolerance using different physiological and biochemical indexes. Dehydration-responsive temporal changes of ECM proteins in JG-62, a relatively tolerant variety, revealed 186 proteins with variance at a 95% significance level statistically. The comparative proteomics analysis led to the identification of 134 differentially expressed proteins that include predicted and novel dehydration-responsive proteins. This study, for the first time, demonstrates that over a hundred ECM proteins, presumably involved in a variety of cellular functions, viz. cell wall modification, signal transduction, metabolism, and cell defense and rescue, impinge on the molecular mechanism of dehydration tolerance in plants.

To whom correspondence may be addressed. Tel.: 91-11-26735178; Fax: 91-11-26716658; E-mail: nchakraborty{at}hotmail.com

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