HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M600336-MCP200v1 6/4/738    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Yokota, H. Articles by Suzuki, H. Search for Related Content PubMed PubMed Citation Articles by Yokota, H. Articles by Suzuki, H. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 6:738-744, 2007.
© 2007 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Absence of Increased ₁-Microglobulin in IgA Nephropathy Proteinuria^*

Hiroyuki Yokota^,, Masashi Hiramoto, Hirokazu Okada^¶, Yoshihiko Kanno^¶, Masatoshi Yuri, Shuji Morita, Masanori Naitou, Atsushi Ichikawa, Masao Katoh and Hiromichi Suzuki^¶

From Astellas Pharm Inc., 21 Miyukigaoka, Tsukuba-shi 305-8585, Japan and the ^¶ Department of Nephrology and Medical Education Center, School of Medicine, Faculty of Medicine, Saitama Medical University, 38 Morohongo, Moroyama, Iruma, Saitama 350-0495, Japan

To search for biomarkers of IgA nephropathy, protein profiles of urine samples from patients with IgA nephropathy and normal volunteers were compared using two-dimensional DIGE. Most of the 172 spots identified in the urine were serum proteins, and their amounts in IgA nephropathy urine were much higher than those in normal urine; this can be explained as proteinuria caused by glomerular dysfunction. However, only ₁-microglobulin, also one of the major serum proteins, in IgA nephropathy urine was not higher in amount than that in normal urine. We confirmed using ELISA analysis that the amounts of transferrin and albumin in IgA nephropathy and diabetic nephropathy urine were much higher than those in normal urine, whereas the amount of ₁-microglobulin in IgA nephropathy urine was not higher than that in normal urine and was much lower than that in diabetic nephropathy urine. Approximately 50% of ₁-microglobulin forms a complex with IgA in serum. These results suggest that ₁-microglobulin in IgA nephropathy urine is a characteristic protein and might be a biomarker for IgA nephropathy and that ₁-microglobulin might have a relationship with IgA nephropathy pathology.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?