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Originally published In Press as doi:10.1074/mcp.R600016-MCP200 on March 20, 2007.
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Molecular & Cellular Proteomics 6:1115-1122, 2007.
© 2007 by The American Society for Biochemistry and Molecular Biology, Inc.

Review

Cancer Immunomics Using Autoantibody Signatures for Biomarker Discovery*

Michel Caron{ddagger},§, Geneviève Choquet-Kastylevsky and Raymonde Joubert-Caron{ddagger}

From the {ddagger} Protein Biochemistry and Proteomics Laboratory, UMR CNRS 7033 (BioMoCeTi), Unité de Formation et de Recherche Santé-Médecine-Biologie Humaine, Paris 13 University, 93017 Bobigny cedex, France and New Markers Department, Advance Technology Unit, bioMérieux, 69280 Marcy-l'Etoile, France

The increased incidence of autoantibodies in malignancies has been described since the 1970s. Thus the ability to determine molecular fingerprinting of autoantibodies (antibody signatures) may provide useful clinical diagnostic and prognostic information. This review describes the use of several proteomics approaches for the identification of antigens recognized by these autoantibodies. Serological proteome analysis combines separation of tumor cell proteins on two-dimensional gel electrophoresis gels, Western blotting with sera of patients and healthy subjects, and identification of the detected antigens by MS. Alternatively multiple affinity protein profiling combines isolation of the antigens recognized by patient antibodies by two-dimensional immunoaffinity chromatography and identification by MS/MS. The use and limitations of reverse phase protein microarrays for testing patient serum containing autoantibodies are also considered. Lastly the most important difficulty of any proteomically identified autoantibody signature is validation in patient cohorts or clinical samples.

§ To whom correspondence should be addressed: Protein Biochemistry and Proteomics Laboratory, CNRS UMR 7033 (BioMoCeTi), UFR SMBH Leonard de Vinci, University Paris 13, 74 rue Marcel Cachin, 93017 Bobigny cedex, France. Tel.: 33-1-48-38-77-54; E-mail: caron_prot{at}yahoo.fr


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[Abstract] [Full Text] [PDF]


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Copyright © 2007 by the American Society for Biochemistry and Molecular Biology.