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Originally published In Press as doi:10.1074/mcp.M700194-MCP200 on November 12, 2007.
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Molecular & Cellular Proteomics 7:431-441, 2008.
© 2008 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Platelet Factor-4 Is an Indicator of Blood Count Recovery in Acute Myeloid Leukemia Patients in Complete Remission*,S

Jin Young Kim{ddagger}, Ho-Jun Song§, Hoi-Jeong Lim, Myung-Geun Shin||, Jae Seong Kim**, Hyeoung-Joon Kim{ddagger}{ddagger}, Baik Yoon Kim** and Seung-won Lee**,§§

From the {ddagger} Proteome Analysis Team, Korea Basic Science Institute, Daejeon 305-333, Korea, Departments of § Dental Materials and Biostatistics, Dental Science Research Institute, Brain Korea 21 Program, Chonnam National University School of Dentistry, Gwangju 501-757, Korea, Departments of || Laboratory Medicine, ** Anatomy, and {ddagger}{ddagger} Internal Medicine, Genome Research Center for Hematopoietic Diseases, Brain Korea 21 Program, Chonnam National University Medical School, Gwangju 501-746, Korea

To investigate whether serum biomarkers can be used to indicate the responsiveness of acute myeloid leukemia to remission induction chemotherapy, we performed MALDI-TOF protein profile analysis of patient sera. The resulting spectra revealed a protein (or peptide) peak at m/z 7764 that varied in intensity; its intensity was much higher in samples from patients in complete remission than in those from patients with resistant disease or in samples taken prior to treatment (at the time of diagnosis). Using fractionation, trypsin digestion, MS/MS, and protein molecular weight analyses, we identified the m/z 7764 protein as platelet factor-4 (PF4). This identification was confirmed by a magnetic bead-based MALDI immunoassay. Statistical comparison of PF4 levels and platelet counts in patient sera revealed a significant positive correlation between the two variables. This study demonstrates that PF4 protein levels are a good indicator for the recovery of blood count in the complete remission of acute myeloid leukemia. The linear positive correlation curve indicates that blood count recovery of platelets to >100,000/mm3 is equivalent to a serum PF4 recovery level of >2.492 µg/ml.

§§ To whom correspondence should be addressed: Dept. of Anatomy, Chonnam National University Medical School, 5 Hakdong, Donggu, Gwangju 501-746, Korea. E-mail: seunglee{at}chonnam.ac.kr


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