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Submitted on January 9, 2002
Revised on March 22, 2002
Accepted on March 25, 2002

A strategy for the rapid identification of phosphorylation sites in the phospho-proteome

Justin A. MacDonald, Aaron J. Mackey, William R. Pearson, and Timothy A.J. Haystead

Pharmacology and Cancer Biology, Duke University, Durham, NC 27710

Corresponding Author: macdo011{at}mc.duke.edu

We have developed a bioinformatic assessment tool, the Cleavage of Radiolabeled Protein program or "CRP-program", which enables experimental identification of phosphorylation sites via 32P labeling and Edman degradation of cleaved proteins obtained at sub-femptomole levels. Given a protein sequence and a cleavage site, CRP performs these experiments in silico, identifying resolved sites based on user-supplied experimental data, as well as suggesting combinations of reagents for additional analysis.


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