| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on February 27, 2003
Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94143
Corresponding Author: alb{at}itsa.ucsf.edu
In order to identify and compare the protein content of very low quantity samples of high complexity, a protocol has been established that combines the differential profiling strength of a new cleavable 13C isotope-coded affinity tag (cICAT) reagent with the high sequence coverage provided by multi-dimensional liquid chromatography and two modes of tandem mass spectrometry. Major objectives during protocol optimization were to minimize sample losses and establish a robust procedure that employs volatile buffer systems that are highly compatible with mass spectrometry. cICAT labeled tryptic peptides were separated from non-labeled peptides by avidin affinity chromatography. Subsequently, peptide samples were analyzed by nanoflow LC-ESI MS/MS and LC-MALDI MS/MS. The use of two ionization/instrumental configurations led to complementary peptide identifications that increased the confidence of protein assignments. Examples that illustrate the power of this strategy are taken from two different projects: (i) immunoaffinity purified complexes containing the prion protein from the murine brain, and (ii) human tracheal epithelium gland secretions. In these studies a large number of novel proteins were identified using stringent match criteria, in addition to many that had been identified in previous experiments. In the latter case the ICAT method produced significant new information on changes that occur in protein expression levels in a patient suffering from cystic fibrosis.
Revised on May 23, 2003
Accepted on May 23, 2003
Mass spectrometric analysis of protein mixtures at low levels using cleavable 13C-ICAT and multi-dimensional chromatography
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Sui, J. Zhang, T. L. Tan, C. B. Ching, and W. N. Chen Comparative Proteomics Analysis of Vascular Smooth Muscle Cells Incubated with S- and R-Enantiomers of Atenolol Using iTRAQ-coupled Two-dimensional LC-MS/MS Mol. Cell. Proteomics, June 1, 2008; 7(6): 1007 - 1018. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wu, Q. Lin, T. K. Lim, T. Liu, and C.-L. Hew White Spot Syndrome Virus Proteins and Differentially Expressed Host Proteins Identified in Shrimp Epithelium by Shotgun Proteomics and Cleavable Isotope-Coded Affinity Tag J. Virol., November 1, 2007; 81(21): 11681 - 11689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. B. McClatchy, L. Liao, S. K. Park, J. D. Venable, and J. R. Yates Quantification of the synaptosomal proteome of the rat cerebellum during post-natal development Genome Res., September 1, 2007; 17(9): 1378 - 1388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Hirsch, K. C. Hansen, A. Sapru, J. A. Frank, R. J. Chalkley, X. Fang, J. C. Trinidad, P. Baker, A. L. Burlingame, and M. A. Matthay Impact of Low and High Tidal Volumes on the Rat Alveolar Epithelial Type II Cell Proteome Am. J. Respir. Crit. Care Med., May 15, 2007; 175(10): 1006 - 1013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-C. Chiang, C.-G. Juo, H.-H. Chang, H.-M. Chen, E. C. Yi, and Y. Chern Systematic Uncovering of Multiple Pathways Underlying the Pathology of Huntington Disease by an Acid-cleavable Isotope-coded Affinity Tag Approach Mol. Cell. Proteomics, May 1, 2007; 6(5): 781 - 797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Moron, N. S. Abul-Husn, R. Rozenfeld, G. Dolios, R. Wang, and L. A. Devi Morphine Administration Alters the Profile of Hippocampal Postsynaptic Density-associated Proteins: A Proteomics Study Focusing on Endocytic Proteins Mol. Cell. Proteomics, January 1, 2007; 6(1): 29 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. P. Vaughn, D. K. Crockett, M. S. Lim, and K. S. J. Elenitoba-Johnson Analytical Characteristics of Cleavable Isotope-Coded Affinity Tag-LC-Tandem Mass Spectrometry for Quantitative Proteomic Studies J. Mol. Diagn., September 1, 2006; 8(4): 513 - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Luo, E. Nieves, J. Kzhyshkowska, and R. H. Angeletti Endogenous Transforming Growth Factor-{beta} Receptor-mediated Smad Signaling Complexes Analyzed by Mass Spectrometry Mol. Cell. Proteomics, July 1, 2006; 5(7): 1245 - 1260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hirsch, K. C. Hansen, S. Choi, J. Noh, R. Hirose, J. P. Roberts, M. A. Matthay, A. L. Burlingame, J. J. Maher, and C. U. Niemann Warm Ischemia-induced Alterations in Oxidative and Inflammatory Proteins in Hepatic Kupffer Cells in Rats Mol. Cell. Proteomics, June 1, 2006; 5(6): 979 - 986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cheng, C. C. Hoogenraad, J. Rush, E. Ramm, M. A. Schlager, D. M. Duong, P. Xu, S. R. Wijayawardana, J. Hanfelt, T. Nakagawa, et al. Relative and Absolute Quantification of Postsynaptic Density Proteome Isolated from Rat Forebrain and Cerebellum Mol. Cell. Proteomics, June 1, 2006; 5(6): 1158 - 1170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Majeran, Y. Cai, Q. Sun, and K. J. van Wijk Functional Differentiation of Bundle Sheath and Mesophyll Maize Chloroplasts Determined by Comparative Proteomics PLANT CELL, November 1, 2005; 17(11): 3111 - 3140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Delcourt, P. Jouin, J. Poncet, E. Demey, E. Mauger, J. Bockaert, P. Marin, and N. Galeotti Difference in Mass Analysis Using Labeled Lysines (DIMAL-K): A New, Efficient Proteomic Quantification Method Applied to the Analysis of Astrocytic Secretomes Mol. Cell. Proteomics, August 1, 2005; 4(8): 1085 - 1094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Chalkley, P. R. Baker, K. C. Hansen, K. F. Medzihradszky, N. P. Allen, M. Rexach, and A. L. Burlingame Comprehensive Analysis of a Multidimensional Liquid Chromatography Mass Spectrometry Dataset Acquired on a Quadrupole Selecting, Quadrupole Collision Cell, Time-of-flight Mass Spectrometer: I. How Much of the Data is Theoretically Interpretable by Search Engines? Mol. Cell. Proteomics, August 1, 2005; 4(8): 1189 - 1193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Chalkley, P. R. Baker, L. Huang, K. C. Hansen, N. P. Allen, M. Rexach, and A. L. Burlingame Comprehensive Analysis of a Multidimensional Liquid Chromatography Mass Spectrometry Dataset Acquired on a Quadrupole Selecting, Quadrupole Collision Cell, Time-of-flight Mass Spectrometer: II. New Developments in Protein Prospector Allow for Reliable and Comprehensive Automatic Analysis of Large Datasets Mol. Cell. Proteomics, August 1, 2005; 4(8): 1194 - 1204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Beynon and J. M. Pratt Metabolic Labeling of Proteins for Proteomics Mol. Cell. Proteomics, July 1, 2005; 4(7): 857 - 872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Wetmore and B. A. Merrick Invited Review: Toxicoproteomics: Proteomics Applied to Toxicology and Pathology Toxicol Pathol, October 1, 2004; 32(6): 619 - 642. [Abstract] [PDF]
|
|
|
|
 |
|
 P. R. Cutillas, R. J. Chalkley, K. C. Hansen, R. Cramer, A. G. W. Norden, M. D. Waterfield, A. L. Burlingame, and R. J. Unwin The urinary proteome in Fanconi syndrome implies specificity in the reabsorption of proteins by renal proximal tubule cells Am J Physiol Renal Physiol, September 1, 2004; 287(3): F353 - F364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Hirsch, K. C. Hansen, A. L. Burlingame, and M. A. Matthay Proteomics: current techniques and potential applications to lung disease Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L1 - L23. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. M. Cristea, S. J. Gaskell, and A. D. Whetton Proteomics techniques and their application to hematology Blood, May 15, 2004; 103(10): 3624 - 3634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, Y. Hong, Y.-X. Tan, H. Zhou, J.-H. Ai, S.-J. Li, L. Zhang, Q.-C. Xia, J.-R. Wu, H.-Y. Wang, et al. Accurate Qualitative and Quantitative Proteomic Analysis of Clinical Hepatocellular Carcinoma Using Laser Capture Microdissection Coupled with Isotope-coded Affinity Tag and Two-dimensional Liquid Chromatography Mass Spectrometry Mol. Cell. Proteomics, April 1, 2004; 3(4): 399 - 409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sethuraman, M. E. McComb, T. Heibeck, C. E. Costello, and R. A. Cohen Isotope-coded Affinity Tag Approach to Identify and Quantify Oxidant-sensitive Protein Thiols Mol. Cell. Proteomics, March 1, 2004; 3(3): 273 - 278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. W. Li, M. P. Hornshaw, R. C. Van der Schors, R. Watson, S. Tate, B. Casetta, C. R. Jimenez, Y. Gouwenberg, E. D. Gundelfinger, K.-H. Smalla, et al. Proteomics Analysis of Rat Brain Postsynaptic Density: IMPLICATIONS OF THE DIVERSE PROTEIN FUNCTIONAL GROUPS FOR THE INTEGRATION OF SYNAPTIC PHYSIOLOGY J. Biol. Chem., January 9, 2004; 279(2): 987 - 1002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Baldwin Protein Identification by Mass Spectrometry: Issues to be Considered Mol. Cell. Proteomics, January 1, 2004; 3(1): 1 - 9. [Full Text] [PDF]
|
|
|
|
|
|
J. V. Olsen, J. R. Andersen, P. Aa. Nielsen, M. L. Nielsen, D. Figeys, M. Mann, and J. R. Wisniewski HysTag--A Novel Proteomic Quantification Tool Applied to Differential Display Analysis of Membrane Proteins From Distinct Areas of Mouse Brain Mol. Cell. Proteomics, January 1, 2004; 3(1): 82 - 92. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Li, H. Steen, and S. P. Gygi Protein Profiling with Cleavable Isotope-coded Affinity Tag (cICAT) Reagents: The Yeast Salinity Stress Response Mol. Cell. Proteomics, November 1, 2003; 2(11): 1198 - 1204. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Journal of Lipid Research | ASBMB Today |
