DIifferential proteomic analysis of the mouse retina: The induction of crystallin proteins by retinal degeneration in the rd1 mouse

doi:10.1074/mcp.M300029-MCP200

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Submitted on March 27, 2003
Revised on June 18, 2003
Accepted on June 26, 2003

DIifferential proteomic analysis of the mouse retina: The induction of crystallin proteins by retinal degeneration in the rd1 mouse

Nükhet Cavusoglu, Danièle Thiersé, Saddek Mohand-Saïd, Frédéric Chalmel, Olivier Poch, Alain van Dorsselaer, José Alain Sahel, and Thierry Léveillard

Ophthalmology, Inserm u 592, Paris, Ile de France 75571

Corresponding Author: leveilla{at}st-antoine.inserm.fr

We have applied proteomic analysis to the degeneration of photoreceptors (PR). In the rd1 mouse a recessive mutation in the PDE6B gene leads to rapid loss of rods through apoptosis. By five weeks postnatal, virtually all rod PR have degenerated leaving one row of cones that degenerates secondarily. In order to assess comparative protein expression, proteins extracted from whole retina were resolved on a 2D gel and identified by mass spectrometry combined with database screening. MALDI-TOF mass spectrometry coupled to peptide mass fingerprinting was sufficient to identify most of the proteins, the remaining being identified with additional sequence information obtained by nanoESI-MS/MS or LC-MS/MS. The study revealed 212 spots, grouped into 109 different proteins. Differential analysis showed loss of proteins involved in the rod-specific phototransduction cascade as well as induction of proteins from the crystallin family, in response to retinal degeneration. Identification of such pathways may contribute to new therapeutic approaches.

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