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Submitted on May 22, 2003
Proteome Sciences plc, Institute of Psychiatry, London SE5 8AF
Corresponding Author: e.mcgregor{at}iop.kcl.ac.uk
Background: Increased force generation and smooth muscle remodelling follow the implantation of saphenous vein as an arterial bypass graft. Previously, we characterised and mapped 129 proteins in human saphenous vein medial smooth muscle, using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Here, we focus on actin filament remodelling in response to simulated arterial flow. Methods and Results: Human saphenous vein was exposed to simulated venous or arterial flow for 90 minutes in vitro, the contractile medial smooth muscle dissected out and subjected to 2-D gel electrophoresis, using a non-linear immobilised pH 3-10 gradient in the first dimension. Proteins were analysed quantitatively using PDQuest 2-D software. The actin polymerisation inhibitor cytochalasin (1
Revised on September 2, 2003
Accepted on November 11, 2003
F-actin capping (CapZ) and other contractile saphenous vein smooth muscle proteins are altered by hemodynamic stress: a proteomic approach
mM) prevented increases in force generation after 90 minutes of simulated arterial flow. At this time point there were several consistent changes in actin filament associated protein expression (7 paired vein samples). The heat shock protein HSP27, identified as a 3-spot charge train, showed a 1.6-fold increase in abundance (p=0.01), but with reduced representation of the phosphorylated Ser82 and Ser15Ser82 isoforms (p=0.018). The abundance of actin-capping protein 
2 subunit CapZ had decreased 3-fold, p=0.04. A 19 kDa proteolytic fragment of actin increased 2-fold, p=0.04. For the 4-spot charge train of gelsolin, there was reduced representation of the more acidic isoforms, p=0.022. The abundance of other proteins associated with actin filaments, including cofilin and destrin, remained unchanged after arterial flow. Conclusions: Actin filament remodelling with differential expression and/or post-translational modification of proteins involved in capping the barbed end of actin filaments, HSP27 and CapZ, is an early response of contractile saphenous vein smooth muscle cells to hemodynamic stress. The observed changes would favour the generation of contractile stress fibres.
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