| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on June 30, 2003
Bioscience Dept., Los Alamos National Lab, Los Alamos, NM 87545
Corresponding Author: chen_xian{at}lanl.gov
Mycobacterium tuberculosis (MTB) is an infectious microorganism that causes human tuberculosis. The cell membrane of a pathogen is known to be rich in diagnostic and therapeutic protein targets. To compliment the MTB genome, we have profiled the membrane protein fraction of the M. tuberculosis H37Rv strain using an analytical platform that couples 1D SDS gels to a microcapillary liquid chromatography-nanospray-tandem mass spectrometer. As a result, 739 proteins has been identified by 2 or more distinct peptide sequences and characterized. Interestingly, approximately 450 proteins represent novel identifications, 79 of which are membrane proteins and more than a hundred are membrane-associated proteins. The physicochemical properties of the identified proteins were studied in detail and then biological functions were obtained by sorting them according Sanger Institute gene function category. Many membrane proteins were found to be involved in the cell envelope and those proteins with energy metabolic functions were also identified in this study
Revised on October 3, 2003
Accepted on October 6, 2003
Comprehensive proteomic profiling of the membrane constituents of A mycobacterium tuberculosis strain
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  W. Shui, L. Sheu, J. Liu, B. Smart, C. J. Petzold, T.-y. Hsieh, A. Pitcher, J. D. Keasling, and C. R. Bertozzi Membrane proteomics of phagosomes suggests a connection to autophagy PNAS, November 4, 2008; 105(44): 16952 - 16957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. McDonough, J. R. McCann, E. M. Tekippe, J. S. Silverman, N. W. Rigel, and M. Braunstein Identification of Functional Tat Signal Sequences in Mycobacterium tuberculosis Proteins J. Bacteriol., October 1, 2008; 190(19): 6428 - 6438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. K. M. Dass, R. Sharma, A. R. Shenoy, R. Mattoo, and S. S. Visweswariah Cyclic AMP in Mycobacteria: Characterization and Functional Role of the Rv1647 Ortholog in Mycobacterium smegmatis J. Bacteriol., June 1, 2008; 190(11): 3824 - 3834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Reinhardt and J. D. Lippolis Developmental Changes in the Milk Fat Globule Membrane Proteome During the Transition from Colostrum to Milk J Dairy Sci, June 1, 2008; 91(6): 2307 - 2318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Chapeton-Montes, D. F. Plaza, H. Curtidor, M. Forero, M. Vanegas, M. E. Patarroyo, and M. A. Patarroyo Characterizing the Mycobacterium tuberculosis Rv2707 protein and determining its sequences which specifically bind to two human cell lines Protein Sci., February 1, 2008; 17(2): 342 - 351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Baharvand, A. Fathi, D. van Hoof, and G. H. Salekdeh Concise Review: Trends in Stem Cell Proteomics Stem Cells, August 1, 2007; 25(8): 1888 - 1903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. S. Gibbons, F. Wolschendorf, M. Abshire, M. Niederweis, and M. Braunstein Identification of Two Mycobacterium smegmatis Lipoproteins Exported by a SecA2-Dependent Pathway J. Bacteriol., July 15, 2007; 189(14): 5090 - 5100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. C. Yeruva, S. Duggirala, V. Lakshmi, D. Kolarich, F. Altmann, and M. Sritharan Identification and Characterization of a Major Cell Wall-Associated Iron-Regulated Envelope Protein (Irep-28) in Mycobacterium tuberculosis Clin. Vaccine Immunol., October 1, 2006; 13(10): 1137 - 1142. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Fischer, D. Wolters, M. Rogner, and A. Poetsch Toward the Complete Membrane Proteome: High Coverage of Integral Membrane Proteins Through Transmembrane Peptide Detection Mol. Cell. Proteomics, March 1, 2006; 5(3): 444 - 453. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Wang, T.-H. Chuang, T. Ronni, S. Gu, Y.-C. Du, H. Cai, H.-Q. Sun, H. L. Yin, and X. Chen Flightless I Homolog Negatively Modulates the TLR Pathway J. Immunol., February 1, 2006; 176(3): 1355 - 1362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sinha, K. Kosalai, S. Arora, A. Namane, P. Sharma, A. N. Gaikwad, P. Brodin, and S. T. Cole Immunogenic membrane-associated proteins of Mycobacterium tuberculosis revealed by proteomics Microbiology, July 1, 2005; 151(7): 2411 - 2419. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. G. Mawuenyega, C. V. Forst, K. M. Dobos, J. T. Belisle, J. Chen, E. M. Bradbury, A. R.M. Bradbury, and X. Chen Mycobacterium tuberculosis Functional Network Analysis by Global Subcellular Protein Profiling Mol. Biol. Cell, January 1, 2005; 16(1): 396 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Carter, S. Pan, J. Zouhar, E. L. Avila, T. Girke, and N. V. Raikhel The Vegetative Vacuole Proteome of Arabidopsis thaliana Reveals Predicted and Unexpected Proteins PLANT CELL, December 1, 2004; 16(12): 3285 - 3303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gu, Z. Liu, S. Pan, Z. Jiang, H. Lu, O. Amit, E. M. Bradbury, C.-A. A. Hu, and X. Chen Global Investigation of p53-induced Apoptosis Through Quantitative Proteomic Profiling Using Comparative Amino Acid-coded Tagging Mol. Cell. Proteomics, October 1, 2004; 3(10): 998 - 1008. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Journal of Lipid Research | ASBMB Today |
